Integrated Metabolomics and Transcriptomics Analyses of the Biosynthesis of Arbutin and 6′-O-Caffeoylarbutin in Vaccinium dunalianum Cell Suspension Cultures Fed with Hydroquinone

doi:10.3390/ijms25147760

KIB OpenIR

	Integrated Metabolomics and Transcriptomics Analyses of the Biosynthesis of Arbutin and 6′-O-Caffeoylarbutin in Vaccinium dunalianum Cell Suspension Cultures Fed with Hydroquinone
	Li, Churan; Wu, Boxiao; Wang, Weihua; Yang, Xiaoqin; Liu, Yun; Zhu, Guolei; Xie, Sida; Jiang, Qian; Ding, Yong; Zhang, Yingjun; Zhao, Ping; Zou, Lihua
	2024
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1661-6596
卷号	25 期号:14 页码:7760
摘要	Arbutin and 6 '-O-caffeoylarbutin (CA) from Vaccinium dunalianum Wight are known for their ability to inhibit melanin synthesis. To boost the production of arbutin and CA, precursor feeding with hydroquinone (HQ) was studied in V. dunalianum suspension cells. The effect of HQ on the biosynthesis of arbutin and CA in the suspension cells was investigated using high-performance liquid chromatography (HPLC), and possible molecular mechanisms were analyzed using metabolomics and transcriptomics analyses. HPLC analysis only showed that the addition of HQ significantly enhanced arbutin synthesis in cells, peaking at 15.52 +/- 0.28 mgg(-1) after 0.5 mmolL-1 HQ treatment for 12 h. Subsequently, metabolomics identified 78 differential expression metabolites (DEMs), of which arbutin and CA were significantly up-regulated metabolites. Moreover, transcriptomics found a total of 10,628 differential expression genes (DEGs). The integrated transcriptomics and metabolomics revealed that HQ significantly enhanced the expression of two arbutin synthase (AS) genes (Unigene0063512 and Unigene0063513), boosting arbutin synthesis. Additionally, it is speculated that CA was generated from arbutin and 3,4,5-tricaffeoylquinic acid catalyzed by caffeoyl transferase, with Unigene0044545, Unigene0043539, and Unigene0017356 as potentially associated genes with CA synthesis. These findings indicate that the precursor feeding strategy offers a promising approach for the mass production of arbutin and CA in V. dunalianum suspension cells and provides new insights for CA biosynthesis in V. dunalianum.
DOI	10.3390/ijms25147760
WOS记录号	WOS:001278657300001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75793
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Li, Churan,Wu, Boxiao,Wang, Weihua,等. Integrated Metabolomics and Transcriptomics Analyses of the Biosynthesis of Arbutin and 6′-O-Caffeoylarbutin in Vaccinium dunalianum Cell Suspension Cultures Fed with Hydroquinone[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2024,25(14):7760.
APA	Li, Churan.,Wu, Boxiao.,Wang, Weihua.,Yang, Xiaoqin.,Liu, Yun.,...&Zou, Lihua.(2024).Integrated Metabolomics and Transcriptomics Analyses of the Biosynthesis of Arbutin and 6′-O-Caffeoylarbutin in Vaccinium dunalianum Cell Suspension Cultures Fed with Hydroquinone.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,25(14),7760.
MLA	Li, Churan,et al."Integrated Metabolomics and Transcriptomics Analyses of the Biosynthesis of Arbutin and 6′-O-Caffeoylarbutin in Vaccinium dunalianum Cell Suspension Cultures Fed with Hydroquinone".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 25.14(2024):7760.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
10.3390_ijms25147760（5472KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览下载