Polysaccharide Peptide Induced Colorectal Cancer Cells Apoptosis by Down-Regulating EGFR and PD-L1 Expression

doi:10.5812/ijpr-123909

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	Polysaccharide Peptide Induced Colorectal Cancer Cells Apoptosis by Down-Regulating EGFR and PD-L1 Expression
	Lin Jian; He Zhicheng; Liu Shubai
	2022
发表期刊	IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH
卷号	21 期号:1 页码:e123909
摘要	Background: Colorectal cancer (CRC) is the most frequent death-causing disease in the world. The Trametes versicolor mushroom, a traditional Chinese medicine, has beenused as anti-cancer medicine with long history. Its cultured mycelia extracts, namelypolysaccharide peptide (PSP) as the major active component in Trametes versicolor, is widely used in eastern countries to stimulate the immune system and treat deadly cancers, including CRC. Methods: This study aimed to explore the mechanism through which PSP inhibits CRC cells proliferation. In vitro, cell proliferation and cytotoxicity of PSP were assessed using human CRC cell lines (HCT116 and HT29). The real-time polymerase chain reaction (PCR), western blot, and immunofluorescence methods were used to examine the expression of epidermal growth factor receptor (EGFR), programmed cell death-ligand 1 (PD-L1), activator of transcription 3 (STAT3), c-Jun, and NF-kappa B in the PSP treated CRC cells. Human peripheral blood mononuclear cells (PBMC), which were activated with CD3/CD28/CD2 T cell activator and interleukin 2 (IL-2), were co-cultured with HCT116, which was pre-treated with PSP to reduce PD-L1 expression. The synergic effect of T-cells killing was evaluated using the terminal-deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) method. Results: Polysaccharide peptide significantly inhibited proliferation of HCT116 and HT29 cell line in vitro. Polysaccharide peptide strongly reduced the expression and phosphorylation level of EGFR. In addition, PSP pretreatment significantly decreased the expression of downstream molecules PD-L1 and EGFR signaling pathways (c-Jun and STAT3) in HCT116. Polysaccharide peptide pretreatment enhanced the T-cells killing effect induced by co-culture PBMC on HCT116 cells. Conclusions: Polysaccharide peptide may be used as a prophylactic and therapeutic agent against CRC via down-regulating PD-L1 and EGFR signaling pathway.
关键词	Colorectal Cancer Trametes Versicolor EGFR PD-L1 Polysaccharide Peptide CORIOLUS-VERSICOLOR SIGNALING PATHWAY COLON-CANCER T-CELLS STATISTICS THERAPY
DOI	10.5812/ijpr-123909
收录类别	SCI
WOS记录号	WOS:000891933200001
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75623
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Lin Jian,He Zhicheng,Liu Shubai. Polysaccharide Peptide Induced Colorectal Cancer Cells Apoptosis by Down-Regulating EGFR and PD-L1 Expression[J]. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH,2022,21(1):e123909.
APA	Lin Jian,He Zhicheng,&Liu Shubai.(2022).Polysaccharide Peptide Induced Colorectal Cancer Cells Apoptosis by Down-Regulating EGFR and PD-L1 Expression.IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH,21(1),e123909.
MLA	Lin Jian,et al."Polysaccharide Peptide Induced Colorectal Cancer Cells Apoptosis by Down-Regulating EGFR and PD-L1 Expression".IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH 21.1(2022):e123909.

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