COMPARATIVE SEED CRYOPRESERVATION OF INDONESIAN AND NEW ZEALAND EPIPHYTIC AND TERRESTRIAL ORCHIDS

doi:10.54680/fr23410110312

KIB OpenIR

	COMPARATIVE SEED CRYOPRESERVATION OF INDONESIAN AND NEW ZEALAND EPIPHYTIC AND TERRESTRIAL ORCHIDS
	Diantina,Surya; McGill,Craig; McCormick,Andrea Clavijo; Millner,James; Pritchard,Hugh W.; Nadarajan,Jayanthi
	2023
发表期刊	CRYOLETTERS
ISSN	1742-0644
卷号	44 期号:4 页码:197-207
摘要	BACKGROUND: The atypical seed storage behaviour reported in several orchid species justifies cryopreservation as a complementary conservation strategy to conventional seed banking. OBJECTIVE: This study aimed to assess the seed cryopreservation potential of five orchid species; two tropical epiphytic, Indonesian species (Dendrobium strebloceras, D. lineale), one temperate epiphytic, New Zealand species (D. cunninghamii) and two temperate terrestrial, New Zealand species (Pterostylis banksii, Thelymitra nervosa). MATERIALS AND METHODS: Seeds were cryopreserved by direct immersion in liquid nitrogen (LN) and through the application of a cryoprotectant vitrification method. For the latter, seeds were exposed to Plant Vitrification Solution 2 (PVS2) for 0, 20, 50, and 70 min, at either room temperature or on ice, prior to immersion in LN. RESULTS: Seeds of all the studied species germinated well following direct cooling in LN. There was no difference in the seedling development capability between cryopreserved and non-cryopreserved seeds of both tropical epiphytic species and direct immersion in LN enhanced seed germination and shoot formation in both temperate terrestrials. CONCLUSION: Through a range of analyses of germination and postgermination growth, our study shows the potential for cryopreserving epiphytic or terrestrial orchids from tropical and temperate regions.
关键词	cryobiotechnology ex situ conservation direct cooling seed storage temperate species tropical species vitrification CONSERVATION GERMINATION LONGEVITY STORAGE CRYOBIOTECHNOLOGY PROTOCOLS
DOI	10.54680/fr23410110312
收录类别	SCI
WOS记录号	WOS:001167715500004
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75619
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Diantina,Surya,McGill,Craig,McCormick,Andrea Clavijo,et al. COMPARATIVE SEED CRYOPRESERVATION OF INDONESIAN AND NEW ZEALAND EPIPHYTIC AND TERRESTRIAL ORCHIDS[J]. CRYOLETTERS,2023,44(4):197-207.
APA	Diantina,Surya,McGill,Craig,McCormick,Andrea Clavijo,Millner,James,Pritchard,Hugh W.,&Nadarajan,Jayanthi.(2023).COMPARATIVE SEED CRYOPRESERVATION OF INDONESIAN AND NEW ZEALAND EPIPHYTIC AND TERRESTRIAL ORCHIDS.CRYOLETTERS,44(4),197-207.
MLA	Diantina,Surya,et al."COMPARATIVE SEED CRYOPRESERVATION OF INDONESIAN AND NEW ZEALAND EPIPHYTIC AND TERRESTRIAL ORCHIDS".CRYOLETTERS 44.4(2023):197-207.