COMPARATIVE CRYOPRESERVATION OF INDIAN WILD ORANGE (Citrus indica Tanaka) EMBRYONIC AXES

doi:10.54680/fr23310110512

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	COMPARATIVE CRYOPRESERVATION OF INDIAN WILD ORANGE (Citrus indica Tanaka) EMBRYONIC AXES
	Malik,S. K.; Kaur,Sukhdeep; Choudhary,Ravish; Chaudhury,Rekha; Pritchard,Hugh W.
	2023
发表期刊	CRYOLETTERS
ISSN	1742-0644
卷号	44 期号:3 页码:142-150
摘要	BACKGROUND: Indian Wild Orange (Citrus indica Tanaka) is an endangered and endemic species from northeast India for which effective ex situ conservation strategies, including embryo cryopreservation, are urgently needed. MATERIALS AND METHODS: Desiccation tolerance and cryopreservation ability for embryonic axes of Citrus indica was determined using three techniques (air desiccation-freezing, PVS2 vitrification-freezing and encapsulation-dehydration-freezing). Success was assessed as survival and recovery in vitro. RESULTS: Successful cryopreservation of embryonic axes was achieved using all three methods, with the highest survival achieved when using air desiccationfreezing (90%) followed by encapsulation-dehydration (85%) and PVS2 vitrification cryopreservation (80%). Regeneration levels were lower than survival levels for all three proceedures. Post-cryo regeneration success was: encapsulation-dehydration (64%) > air desiccation-freezing (55%) > PVS2 vitrification (52%). CONCLUSION: Although there was relatively high post-cryopreservation recovery growth obtained using all the three techniques, the air desiccation-freezing technique is preferred, as it is a simple, practical and reproducible technique for the long-term cryobanking of this important wild species.
关键词	air desiccation encapsulation plant conservation pre-culture vitrification NUCELLAR CELLS SINENSIS OSB BEHAVIOR SEEDS
学科领域	Life Sciences & Biomedicine - Other Topics ; Physiology
DOI	10.54680/fr23310110512
收录类别	SCI
WOS记录号	WOS:001013076700003
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75618
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Malik,S. K.,Kaur,Sukhdeep,Choudhary,Ravish,et al. COMPARATIVE CRYOPRESERVATION OF INDIAN WILD ORANGE (Citrus indica Tanaka) EMBRYONIC AXES[J]. CRYOLETTERS,2023,44(3):142-150.
APA	Malik,S. K.,Kaur,Sukhdeep,Choudhary,Ravish,Chaudhury,Rekha,&Pritchard,Hugh W..(2023).COMPARATIVE CRYOPRESERVATION OF INDIAN WILD ORANGE (Citrus indica Tanaka) EMBRYONIC AXES.CRYOLETTERS,44(3),142-150.
MLA	Malik,S. K.,et al."COMPARATIVE CRYOPRESERVATION OF INDIAN WILD ORANGE (Citrus indica Tanaka) EMBRYONIC AXES".CRYOLETTERS 44.3(2023):142-150.

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