Tryptophan-centered metabolic alterations coincides with lipid-mediated fungal response to cold stress

doi:10.1016/j.heliyon.2023.e13066

KIB OpenIR

	Tryptophan-centered metabolic alterations coincides with lipid-mediated fungal response to cold stress
	Chen,Yonghong; Yang,Xiaoyu; Zhang,Longlong; Wu,Qunfu; Li,Shuhong; Gou,Jianghui; He,Jiangbo; Zhang,Keqin; Li,Shenghong; Niu,Xuemei
	2023
发表期刊	HELIYON
ISSN	2405-8440
卷号	9 期号:2 页码:e13066
摘要	Tryptophan and its derived metabolites have been assumed to play important roles in the development and survival of organisms. However, the links of tryptophan and its derived metabolites to temperature change remained largely cryptic. Here we presented that a class of prenyl indole alkaloids biosynthesized from tryptophan dramatically accumulated in thermophilic fungus Thermomyces dupontii under cold stress, in which lipid droplets were also highly accumulated and whose conidiophores were highly build-up. Concurrently, disruption of the key NRPS gene involved in the biosynthesis of prenyl indole alkaloids, resulted in decreased lipid and shrunken mitochondria but enlarged vacuoles. Moreover, the Fe3+ and superoxide levels in Delta NRPS were significantly increased but the reactive oxygen species lipid peroxidation and autophagy levels decreased. Metabolomics study revealed that most enriched metabolites in Delta NRPS were mainly composed of tryptophan degraded metabolites including well known ROS scavenger kynurenamines, and lipid-inhibitors, anthranilic acid and indoleacetic acid, and free radical reaction suppressor free fatty acids. Transcriptomic analysis suggested that the key gene involved in tryptophan metabolism, coinciding with the lipid metabolic processes and ion transports were most up-regulated in Delta NRPS under stress. Our results confirmed a lipid-mediated fungal response to cold stress and unveiled a link of tryptophan-based metabolic reprogramming to the fungal cold adaption.
关键词	Thermomyces dupontii Prenylated indole alkaloids Tryptophan Lipids Cold stress Tryptophan metabolism PRENYLATED INDOLE ALKALOIDS SECONDARY METABOLISM THERMOPHILIC FUNGI PEROXIDATION DIVERSITY
学科领域	Science & Technology - Other Topics
DOI	10.1016/j.heliyon.2023.e13066
收录类别	SCI
WOS记录号	WOS:000969447800001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75556
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Chen,Yonghong,Yang,Xiaoyu,Zhang,Longlong,et al. Tryptophan-centered metabolic alterations coincides with lipid-mediated fungal response to cold stress[J]. HELIYON,2023,9(2):e13066.
APA	Chen,Yonghong.,Yang,Xiaoyu.,Zhang,Longlong.,Wu,Qunfu.,Li,Shuhong.,...&Niu,Xuemei.(2023).Tryptophan-centered metabolic alterations coincides with lipid-mediated fungal response to cold stress.HELIYON,9(2),e13066.
MLA	Chen,Yonghong,et al."Tryptophan-centered metabolic alterations coincides with lipid-mediated fungal response to cold stress".HELIYON 9.2(2023):e13066.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
1-s2.0-S240584402300（14809KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览下载