Microbiota-derived short-chain fatty acids mediate the effects of dengzhan shengmai in ameliorating cerebral ischemia via the gut-brain axis

doi:10.1016/j.jep.2023.116158

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	Microbiota-derived short-chain fatty acids mediate the effects of dengzhan shengmai in ameliorating cerebral ischemia via the gut-brain axis
	Guo,Hui-Hui; Shen,Hao-Ran; Tang,Ming-Ze; Sheng,Ning; Ding,Xiao; Lin,Yuan; Zhang,Jin-Lan; Jiang,Jian-Dong; Gao,Tian-Le; Wang,Lu-Lu; Han,Yan-Xing
	2023
发表期刊	JOURNAL OF ETHNOPHARMACOLOGY
ISSN	1872-7573
卷号	306 页码:116158
摘要	Ethnopharmacological relevance: Dengzhan shengmai (DZSM) formula, composed of four herbal medicines (Erigeron breviscapus, Panax ginseng, Schisandra chinensis, and Ophiopogon japonicus), is widely used in the re-covery period of ischemic cerebrovascular diseases; however, the associated molecular mechanism remains unclear.Aim of the study: The purpose of this study was to uncover the links between the microbiota-gut-brain axis and the efficacy of DZSM in ameliorating cerebral ischemic diseases. Materials and methods: The effects of DZSM on the gut microbiota community and bacteria-derived short-chain fatty acid (SCFA) production were evaluated in vivo using a rat model of cerebral ischemia and in vitro through the anaerobic incubation with fresh feces derived from model animals. Subsequently, the mechanism underlying the role of SCFAs in the DZSM-mediated treatment of cerebral ischemia was explored. Results: We found that DZSM treatment significantly altered the composition of the gut microbiota and markedly enhanced SCFA production. The consequent increase in SCFA levels led to the upregulation of the expression of monocarboxylate transporters and facilitated the transportation of intestinal SCFAs into the brain, thereby inhibiting the apoptosis of neurocytes via the regulation of the PI3K/AKT/caspase-3 pathway. The increased intestinal SCFA levels also contributed to the repair of the 2VO-induced disruption of gut barrier integrity and inhibited the translocation of lipopolysaccharide from the intestine to the brain, thus attenuating neuro-inflammation. Consequently, cerebral neuropathy and oxidative stress were significantly improved in 2VO model rats, leading to the amelioration of cerebral ischemia-induced cognitive dysfunction. Finally, fecal microbiota transplantation could reproduce the beneficial effects of DZSM on SCFA production and cerebral ischemia.Conclusions: Our findings suggested that SCFAs mediate the effects of DZSM in ameliorating cerebral ischemia via the gut microbiota-gut-brain axis.
关键词	Dengzhan shengmai Microbiota-gut-brain axis Short-chain fatty acids Cerebral ischemia Gut barrier Fecal microbiota transplantation STROKE SCUTELLARIN
学科领域	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
DOI	10.1016/j.jep.2023.116158
收录类别	SCI
WOS记录号	WOS:000921382700001
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75502
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Guo,Hui-Hui,Shen,Hao-Ran,Tang,Ming-Ze,et al. Microbiota-derived short-chain fatty acids mediate the effects of dengzhan shengmai in ameliorating cerebral ischemia via the gut-brain axis[J]. JOURNAL OF ETHNOPHARMACOLOGY,2023,306:116158.
APA	Guo,Hui-Hui.,Shen,Hao-Ran.,Tang,Ming-Ze.,Sheng,Ning.,Ding,Xiao.,...&Han,Yan-Xing.(2023).Microbiota-derived short-chain fatty acids mediate the effects of dengzhan shengmai in ameliorating cerebral ischemia via the gut-brain axis.JOURNAL OF ETHNOPHARMACOLOGY,306,116158.
MLA	Guo,Hui-Hui,et al."Microbiota-derived short-chain fatty acids mediate the effects of dengzhan shengmai in ameliorating cerebral ischemia via the gut-brain axis".JOURNAL OF ETHNOPHARMACOLOGY 306(2023):116158.

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