Metabolic and transcriptomic analyses elucidate a novel insight into the network for biosynthesis of carbohydrate and secondary metabolites in the stems of a medicinal orchid Dendrobium nobile

doi:10.1016/j.pld.2022.10.004

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	Metabolic and transcriptomic analyses elucidate a novel insight into the network for biosynthesis of carbohydrate and secondary metabolites in the stems of a medicinal orchid Dendrobium nobile
	Zhang,Yu-Wen; Shi,Yu-Cen; Zhang,Shi-Bao
	2023
发表期刊	PLANT DIVERSITY
ISSN	2468-2659
卷号	45 期号:3 页码:326-336
摘要	Dendrobium nobile is an important medicinal and nutraceutical herb. Although the ingredients of D. nobile have been identified as polysaccharides, alkaloids, amino acids, flavonoids and bibenzyls, our understanding of the metabolic pathways that regulate the synthesis of these compounds is limited. Here, we used transcriptomic and metabolic analyses to elucidate the genes and metabolites involved in the biosynthesis of carbohydrate and several secondary metabolites in the stems of D. nobile. A total of 1005 metabolites and 31,745 genes were detected in the stems of D. nobile. The majority of these me-tabolites and genes were involved in the metabolism of carbohydrates (fructose, mannose, glucose, xylulose and starch), while some were involved in the metabolism of secondary metabolites (alkaloids, b-tyrosine, ferulic acid, 4-hydroxybenzoate and chrysin). Our predicted regulatory network indicated that five genes (AROG, PYK, DXS, ACEE and HMGCR) might play vital roles in the transition from carbohydrate to alkaloid synthesis. Correlation analysis identified that six genes (ALDO, PMM, BGLX, EGLC, XYLB and GLGA) were involved in carbohydrate metabolism, and two genes (ADT and CYP73A) were involved in secondary metabolite biosynthesis. Our analyses also indicated that phosphoenol-pyruvate (PEP) was a crucial bridge that connected carbohydrate to alkaloid biosynthesis. The regulatory network between carbohydrate and secondary metabolite biosynthesis established will provide important insights into the regulation of metabolites and biological systems in Dendrobium species. Copyright & COPY; 2022 Kunming Institute of Botany, Chinese Academy of Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
关键词	Dendrobium nobile Transcriptome Metabolome Polysaccharides Alkaloids Secondary metabolite biosynthesis POLYSACCHARIDES GENES OFFICINALE IDENTIFICATION TIME
学科领域	Plant Sciences
DOI	10.1016/j.pld.2022.10.004
收录类别	SCI
WOS记录号	WOS:001017217100001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75386
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Zhang,Yu-Wen,Shi,Yu-Cen,Zhang,Shi-Bao. Metabolic and transcriptomic analyses elucidate a novel insight into the network for biosynthesis of carbohydrate and secondary metabolites in the stems of a medicinal orchid Dendrobium nobile[J]. PLANT DIVERSITY,2023,45(3):326-336.
APA	Zhang,Yu-Wen,Shi,Yu-Cen,&Zhang,Shi-Bao.(2023).Metabolic and transcriptomic analyses elucidate a novel insight into the network for biosynthesis of carbohydrate and secondary metabolites in the stems of a medicinal orchid Dendrobium nobile.PLANT DIVERSITY,45(3),326-336.
MLA	Zhang,Yu-Wen,et al."Metabolic and transcriptomic analyses elucidate a novel insight into the network for biosynthesis of carbohydrate and secondary metabolites in the stems of a medicinal orchid Dendrobium nobile".PLANT DIVERSITY 45.3(2023):326-336.

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