Fengycin produced by Bacillus subtilis XF-1 plays a major role in the biocontrol of Chinese cabbage clubroot via direct effect and defense stimulation

doi:10.1002/jcp.30991

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	Fengycin produced by Bacillus subtilis XF-1 plays a major role in the biocontrol of Chinese cabbage clubroot via direct effect and defense stimulation
	He,Pengjie; Cui,Wenyan; Munir,Shahzad; He,Pengbo; Huang,Ruirong; Li,Xingyu; Wu,Yixin; Wang,Yuehu; Yang,Jing; Tang,Ping; He,Yueqiu; He,Pengfei
	2023
发表期刊	JOURNAL OF CELLULAR PHYSIOLOGY
ISSN	1097-4652
摘要	Bacillus subtilis XF-1 is a well-investigated biocontrol agent against the biotrophic Plasmodiophora brassicae Woron., the causal agent of clubroot disease of cruciferous crops. The present study demonstrates that XF-1 could efficiently control clubroot disease via leaf spraying and provides an understanding of the biocontrol mechanisms. High-performance thin-layer chromatography (HTPLC) analysis indicated the presence of fengycin-type cyclopeptides in the supernatant. A ppsB deletion mutant of XF-1 resulted in no fengycin production, significantly reduced the lysis rate of testing spores in vitro and the primary infection rate of root hair in vivo, and decreased the protection value against clubroot disease under the greenhouse conditions. Confocal laser scanning microscopy proved that fengycin was not required for leaf internalization and root colonization. Moreover, the expression level of the ppsB gene in XF-1 was regulated by its cell density in root during interaction with P. brassicae. In addition, the Delta ppsB mutant of XF-1 could not efficiently control disease because it led to a lower activation level of the jasmonic acid and salicylic acid signaling pathways in roots, which are necessary for the plant defense reaction upon pathogen invasion. Altogether, the present study provides a new understanding of specific cues in the interaction between B. subtilis and P. brassicae as well as insights into the application of B. subtilis in agriculture.
关键词	Bacillus subtilis colonization fengycin foliar application Plasmodiophora brassicae Woron soil-borne disease PLASMODIOPHORA-BRASSICAE BACTERIAL WILT LIPOPEPTIDES SURFACTIN ANTIBIOTICS RESISTANCE DISEASE ITURIN
学科领域	Cell Biology ; Physiology
DOI	10.1002/jcp.30991
收录类别	SCI
WOS记录号	WOS:000951060700001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75313
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	He,Pengjie,Cui,Wenyan,Munir,Shahzad,et al. Fengycin produced by Bacillus subtilis XF-1 plays a major role in the biocontrol of Chinese cabbage clubroot via direct effect and defense stimulation[J]. JOURNAL OF CELLULAR PHYSIOLOGY,2023.
APA	He,Pengjie.,Cui,Wenyan.,Munir,Shahzad.,He,Pengbo.,Huang,Ruirong.,...&He,Pengfei.(2023).Fengycin produced by Bacillus subtilis XF-1 plays a major role in the biocontrol of Chinese cabbage clubroot via direct effect and defense stimulation.JOURNAL OF CELLULAR PHYSIOLOGY.
MLA	He,Pengjie,et al."Fengycin produced by Bacillus subtilis XF-1 plays a major role in the biocontrol of Chinese cabbage clubroot via direct effect and defense stimulation".JOURNAL OF CELLULAR PHYSIOLOGY (2023).

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