Bacillus subtilis YZ-1 surfactins are involved in effective toxicity against agricultural pests

doi:10.1002/ps.7759

KIB OpenIR

	Bacillus subtilis YZ-1 surfactins are involved in effective toxicity against agricultural pests
	Xia,Mengyuan; Munir,Shahzad; Li,Yongmei; Ahmed,Ayesha; He,Pengbo; Wu,Yixin; Li,Xingyu; Tang,Ping; Wang,Zaiqiang; He,Pengfei; Wang,Yuehu; He,Yueqiu
	2023
发表期刊	PEST MANAGEMENT SCIENCE
ISSN	1526-4998
摘要	BACKGROUND: Insect pests negatively affect crop quality and yield. The excessive use of chemical pesticides has serious impacts on the environment and food safety. Therefore, development of effective management strategies in the form of bio-agents have important agricultural applications. Tenebrio molitor, a storage pest, causes losses of grains, medicinal materials, and various agricultural and related products in the warehouse. Bacillus subtilis YZ-1 isolated from naturally deceased Pieris rapae has been found to exhibit significant toxicity against T. molitor.RESULTS: Treatment with B. subtilis YZ-1 fermentation broth resulted in a 90-95% mortality rate of T. molitor within 36 h post-treatment, indicating some active substances may have insecticidal activity in the bacterial supernatant. A bioactivity-guided fractionation method was used to isolate the insecticidal compounds from YZ-1, which led to the identification of surfactins. Additionally, a surfactin deletion mutant YZ-1 Delta srfAA was constructed and the surfactin production by the mutant YZ-1 Delta srfAA was verified through liquid chromatography-mass spectrometry (LC-MS). Further, YZ-1 Delta srfAA exhibited loss of insecticidal activity against T. molitor, Plutella xylostella and Achelura yunnanensis. The insecticidal activity and surfactins contents of several strains of Bacillus sp. were also tested and correlation was found between varying surfactins yield and insecticidal activity exhibited by different strains.CONCLUSION: Conclusively, our results suggest that B. subtilis YZ-1 may provide a novel approach for plant protection against agricultural pests.(c) 2023 Society of Chemical Industry.
关键词	Bacillus Tenebrio molitor agricultural pests surfactins bioinsecticide CYCLIC LIPOPEPTIDES SPB1 BIOSURFACTANT GENOME LEPIDOPTERA IDENTIFICATION SPORULATION METABOLITES SEQUENCE MIDGUT
DOI	10.1002/ps.7759
收录类别	SCI
WOS记录号	WOS:001074833400001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75310
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Xia,Mengyuan,Munir,Shahzad,Li,Yongmei,et al. Bacillus subtilis YZ-1 surfactins are involved in effective toxicity against agricultural pests[J]. PEST MANAGEMENT SCIENCE,2023.
APA	Xia,Mengyuan.,Munir,Shahzad.,Li,Yongmei.,Ahmed,Ayesha.,He,Pengbo.,...&He,Yueqiu.(2023).Bacillus subtilis YZ-1 surfactins are involved in effective toxicity against agricultural pests.PEST MANAGEMENT SCIENCE.
MLA	Xia,Mengyuan,et al."Bacillus subtilis YZ-1 surfactins are involved in effective toxicity against agricultural pests".PEST MANAGEMENT SCIENCE (2023).

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