Sesquiterpenoids from Alpinia oxyphylla with GLP-1 Stimulative Effects through Ca2+/CaMKII and PKA Pathways and Multiple-Enzyme Inhibition

doi:10.1021/acs.jafc.3c06093

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	Sesquiterpenoids from Alpinia oxyphylla with GLP-1 Stimulative Effects through Ca2+/CaMKII and PKA Pathways and Multiple-Enzyme Inhibition
	Cui,Can; Wu,Sheng-Li; Chen,Ji-Jun; Gongpan,Pianchou; Guan,Min; Geng,Chang-An
	2023
发表期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN	1520-5118
卷号	71 期号:43 页码:16148-16159
摘要	Six new sesquiterpenoids (1-6), a pair of enantiomers (7a and 7b), and six known ones (8-13) were isolated from the fruits of Alpinia oxyphylla. The structures of the new compounds were elucidated by extensive spectroscopic data and ECD calculations. The stereochemistry of 7a and 7b was reported for the first time. All compounds showed significant GLP-1 stimulation in NCI-H716 cells with promoting ratios ranging from 90.4 to 668.9% at 50 mu M. Mechanism study indicated that compound 6 stimulated GLP-1 secretion mainly by regulating the transcription and the shearing process of proglucagon, while compound 13 exerted its effects through up-regulating prkaca levels. Interestingly, the GLP-1 stimulative effects of 6 and 13 were both closely related with Ca2+/CaMKII and PKA pathways but irrelevant to TGR5 and GPR119 receptors. Moreover, most compounds exhibited inhibitory activity against alpha-glucosidase and PTP1B at concentrations of 100 and 200 mu M, while showing no activity against GPa. Compounds 3, 9, 11, and 13 could suppress alpha-glucosidase with IC50 values of 190.0, 204.0, 181.8, and 159.6 mu M, equivalent to acarbose (IC50 = 212.0 mu M). This study manifests that A. oxyphylla contains diverse sesquiterpenoids with multiple activities.
关键词	Alpinia oxyphylla sesquiterpenoids GLP-1 stimulation Ca2+/CaMKII and PKA pathways alpha-glucosidase and PTP1B inhibitory activity
DOI	10.1021/acs.jafc.3c06093
收录类别	SCI
WOS记录号	WOS:001097005900001
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75296
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Cui,Can,Wu,Sheng-Li,Chen,Ji-Jun,et al. Sesquiterpenoids from Alpinia oxyphylla with GLP-1 Stimulative Effects through Ca2+/CaMKII and PKA Pathways and Multiple-Enzyme Inhibition[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2023,71(43):16148-16159.
APA	Cui,Can,Wu,Sheng-Li,Chen,Ji-Jun,Gongpan,Pianchou,Guan,Min,&Geng,Chang-An.(2023).Sesquiterpenoids from Alpinia oxyphylla with GLP-1 Stimulative Effects through Ca2+/CaMKII and PKA Pathways and Multiple-Enzyme Inhibition.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,71(43),16148-16159.
MLA	Cui,Can,et al."Sesquiterpenoids from Alpinia oxyphylla with GLP-1 Stimulative Effects through Ca2+/CaMKII and PKA Pathways and Multiple-Enzyme Inhibition".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 71.43(2023):16148-16159.

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