Sesquiterpenoids from Alpinia oxyphylla with GLP-1 Stimulative Effects through Ca2+/CaMKII and PKA Pathways and Multiple-Enzyme Inhibition

doi:10.1021/acs.jafc.3c06093

KIB OpenIR

	Sesquiterpenoids from Alpinia oxyphylla with GLP-1 Stimulative Effects through Ca2+/CaMKII and PKA Pathways and Multiple-Enzyme Inhibition
	Cui,Can; Wu,Sheng-Li; Chen,Ji-Jun; Gongpan,Pianchou; Guan,Min; Geng,Chang-An
	2023
Source Publication	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN	1520-5118
Volume	71 Issue:43 Pages:16148-16159
Abstract	Six new sesquiterpenoids (1-6), a pair of enantiomers (7a and 7b), and six known ones (8-13) were isolated from the fruits of Alpinia oxyphylla. The structures of the new compounds were elucidated by extensive spectroscopic data and ECD calculations. The stereochemistry of 7a and 7b was reported for the first time. All compounds showed significant GLP-1 stimulation in NCI-H716 cells with promoting ratios ranging from 90.4 to 668.9% at 50 mu M. Mechanism study indicated that compound 6 stimulated GLP-1 secretion mainly by regulating the transcription and the shearing process of proglucagon, while compound 13 exerted its effects through up-regulating prkaca levels. Interestingly, the GLP-1 stimulative effects of 6 and 13 were both closely related with Ca2+/CaMKII and PKA pathways but irrelevant to TGR5 and GPR119 receptors. Moreover, most compounds exhibited inhibitory activity against alpha-glucosidase and PTP1B at concentrations of 100 and 200 mu M, while showing no activity against GPa. Compounds 3, 9, 11, and 13 could suppress alpha-glucosidase with IC50 values of 190.0, 204.0, 181.8, and 159.6 mu M, equivalent to acarbose (IC50 = 212.0 mu M). This study manifests that A. oxyphylla contains diverse sesquiterpenoids with multiple activities.
Keyword	Alpinia oxyphylla sesquiterpenoids GLP-1 stimulation Ca2+/CaMKII and PKA pathways alpha-glucosidase and PTP1B inhibitory activity
DOI	10.1021/acs.jafc.3c06093
Indexed By	SCI
WOS ID	WOS:001097005900001
Citation statistics
Document Type	期刊论文
Identifier	http://ir.kib.ac.cn/handle/151853/75296
Collection	中国科学院昆明植物研究所
Recommended Citation GB/T 7714	Cui,Can,Wu,Sheng-Li,Chen,Ji-Jun,et al. Sesquiterpenoids from Alpinia oxyphylla with GLP-1 Stimulative Effects through Ca2+/CaMKII and PKA Pathways and Multiple-Enzyme Inhibition[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2023,71(43):16148-16159.
APA	Cui,Can,Wu,Sheng-Li,Chen,Ji-Jun,Gongpan,Pianchou,Guan,Min,&Geng,Chang-An.(2023).Sesquiterpenoids from Alpinia oxyphylla with GLP-1 Stimulative Effects through Ca2+/CaMKII and PKA Pathways and Multiple-Enzyme Inhibition.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,71(43),16148-16159.
MLA	Cui,Can,et al."Sesquiterpenoids from Alpinia oxyphylla with GLP-1 Stimulative Effects through Ca2+/CaMKII and PKA Pathways and Multiple-Enzyme Inhibition".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 71.43(2023):16148-16159.

Files in This Item:		Download All
File Name/Size	DocType	Version	Access	License
10.1021_acs.jafc.3c0（2550KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	View Download

File name:	10.1021_acs.jafc.3c06093.pdf
Format:	Adobe PDF

If you have any objections to this item, please fill out the form below and the administrator will contact you as soon as possible.
Content:
Email：	*
Institution:
Verification Code:	Refresh

Any comments and suggestions are welcomed.
Title:	*
Content:
Email：	*
Verification Code:	Refresh

中国科学院昆明植物研究所知识管理系统