Marsdenia tenacissima genome reveals calcium adaptation and tenacissoside biosynthesis

doi:10.1111/tpj.16081

KIB OpenIR

	Marsdenia tenacissima genome reveals calcium adaptation and tenacissoside biosynthesis
	Zhou,Yanli; Fan,Wei; Zhang,Haoyue; Zhang,Jingling; Zhang,Guanghui; Wang,Ding; Xiang,Guisheng; Zhao,Changhong; Li,Lianhua; He,Simei; Lu,Yingchun; Zhao,Jiuxia; Meng,Zhengui; Zhang,Xianmin; Meng,Hengling; Yin,Xinhua; Yang,Shengchao; Long,Guangqiang
	2023
Source Publication	PLANT JOURNAL
ISSN	1365-313X
Volume	113 Issue:6 Pages:1146-1159
Abstract	Marsdenia tenacissima is a medicinal plant widely distributed in the calcium-rich karst regions of southwest China. However, the lack of a reference genome has hampered the implementation of molecular techniques in its breeding, pharmacology and domestication. We generated the chromosome-level genome assembly in Apocynaceae using combined SMRT sequencing and Hi-C. The genome length was 381.76 Mb, with 98.9% of it found on 11 chromosomes. The genome contained 222.63 Mb of repetitive sequences and 21 899 predicted gene models, with a contig N50 of 6.57 Mb. Phylogenetic analysis revealed that M. tenacissima diverged from Calotropis gigantea at least 13.43 million years ago. Comparative genomics showed that M. tenacissima underwent ancient shared whole-genome duplication. This event, together with tandem duplication, contributed to 70.71% of gene-family expansion. Both pseudogene analysis and selective pressure calculations suggested calcium-related adaptive evolution in the M. tenacissima genome. Calcium-induced differentially expressed genes (DEGs) were mainly enriched in cell-wall-related processes. Domains (e.g. Fasciclin and Amb_all) and cis-elements (e.g. MYB and MYC) frequently occurred in the coding and promoter regions of cell-wall DEGs, respectively, and the expression levels of these genes correlated significantly with those of calcium-signal-related transcription factors. Moreover, calcium addition increased tenacissoside I, G and H contents. The availability of this high-quality genome provides valuable genomic information for genetic breeding and molecular design, and lends insights into the calcium adaptation of M. tenacissima in karst areas.
Keyword	genome assembly Hi-C Apocynaceae Ca2+ adaptation cell-wall remodeling Marsdenia tenacissima CELL GENES TRANSCRIPTOME MECHANISMS ALIGNMENT SOIL
Subject Area	Plant Sciences
DOI	10.1111/tpj.16081
Indexed By	SCI
WOS ID	WOS:000937341900001
Citation statistics
Document Type	期刊论文
Identifier	http://ir.kib.ac.cn/handle/151853/75249
Collection	中国科学院昆明植物研究所
Recommended Citation GB/T 7714	Zhou,Yanli,Fan,Wei,Zhang,Haoyue,et al. Marsdenia tenacissima genome reveals calcium adaptation and tenacissoside biosynthesis[J]. PLANT JOURNAL,2023,113(6):1146-1159.
APA	Zhou,Yanli.,Fan,Wei.,Zhang,Haoyue.,Zhang,Jingling.,Zhang,Guanghui.,...&Long,Guangqiang.(2023).Marsdenia tenacissima genome reveals calcium adaptation and tenacissoside biosynthesis.PLANT JOURNAL,113(6),1146-1159.
MLA	Zhou,Yanli,et al."Marsdenia tenacissima genome reveals calcium adaptation and tenacissoside biosynthesis".PLANT JOURNAL 113.6(2023):1146-1159.

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