不同地区栽培苏铁（Cycas revoluta）珊瑚根内生菌的研究

	不同地区栽培苏铁（Cycas revoluta）珊瑚根内生菌的研究
	刘嘉婷
导师	龚洵
关键词	苏铁，珊瑚根，内生菌，多样性，核心菌群 Cycas revoluta, Coralloid root, Endophyte, Diversity, Core microbiota
摘要	苏铁类植物（Cycads）是目前发现的唯一一类有珊瑚根结构的裸子植物，是探究植物-微生物互作的理想物种。对于苏铁类植物珊瑚根内生菌的研究，大多集中在珊瑚根的形成过程以及内生细菌的组成方面，对珊瑚根内生菌的全面认识尤其是内生真菌方面的研究还很少。目前高通量测序技术的广泛应用，使得系统性地探究苏铁类植物珊瑚根内生菌的组成及多样性成为现实。本研究通过扩增子分析方法对中国13个地区栽培苏铁（Cycas revoluta）珊瑚根内生菌及裸土中微生物的组成和多样性进行了研究，发现苏铁珊瑚根内生菌多样性较高，但各地区间内生菌的组成及多样性差异不显著，表明苏铁与珊瑚根内生菌的共生与栽培地生境无关，此外，RDA分析发现其与地理环境因子也无太大关系。在珊瑚根内生菌及裸土中微生物的系统发育中，蓝细菌门中的Desmonostoc和子囊菌门中的锥毛壳属（Coniochaeta）、Phialemoniopsis均属于优势菌，且位于系统发育树的基部。细菌红球菌属（Rhodococcus）、土壤杆菌属（Edaphobacter）、杆状孢囊菌属（Virgisporangium）、Niastella、Nordella和真菌枝顶孢霉属（Acremonium）、隐球菌属（Cryptococcus）为苏铁珊瑚根内生菌的核心类群。这些菌的存在可能与宿主苏铁的生长、抗胁迫能力有关。在裸土中，细菌芽孢杆菌属（Bacillus）及真菌新赤壳属（Neocosmospora）、篮状菌属（Talaromyces）、镰刀菌属（Fusarium）、Gliocladiopsis、曲霉属（Aspergillus）、螺旋聚孢霉属（Clonostachys）为优势属。本研究为栽培苏铁内生菌及微生物生态学方面的研究提供了理论基础。; Cycads are the only gymnosperms with coralloid roots at present, they are ideal materials to explore the interaction between plants and microorganisms. Most researches about the endophytes within coralloid roots of cycads focused on the formation of coralloid roots and composition of endophytes, while the comprehensive understanding of endophytes, especially endophytic fungi, was rarely studied. At present, the wide application of high-throughput sequencing technology makes it a reality to systematically explore the composition and diversity of endophytes in coralloid roots of cycads. The diversity and composition of microbiota in coralloid roots and bulk soils of cultivated Cycas revoluta collected from thirteen regions of China were analyzed through amplicon analysis. High diversity of endophytes presented in coralloid roots of C. revoluta, while there was no significant difference in composition and diversity of endophytes among different regions, which indicated that the symbiosis between C. revoluta and endophytes within coralloid roots was irrelevant to cultivation area. In addition, redundancy analysis showed that it has little to do with geographical factors. In the phylogenetic analysis of microbiota in coralloid roots and bulk soils, Desmonostoc in cyanobacteria, as well as Coniochaeta and Phialemoniopsis in ascomycota belong to the dominant microbiota in coralloid roots of C. revoluta and are located at the base of phylogenetic tree. Rhodococcus, Edaphobacter, Virgisporangium, Niastella, Nordella for bacteria and Acremonium, Cryptococcus for fungi are identified as core microbiota. The existence of these microbiota may be related to the growth and stress resistance of the host C. revoluta. In bulk soils of coralloid roots, Bacillus for bacteria and Neocosmospora, Talaromyces, Fusarium, Gliocladiopsis, Aspergillus, Clonostachys for fungi are dominant microbiota. The results provided a theoretical reference for researching endophytic fungi and microecology of cultivated C. revoluta.
语种	中文
	2022-05
学位授予单位	中国科学院大学
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75159
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	刘嘉婷. 不同地区栽培苏铁（Cycas revoluta）珊瑚根内生菌的研究[D]. 中国科学院大学,2022.

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刘嘉婷-不同地区栽培苏铁（Cycas r（3103KB）	学位论文		限制开放	CC BY-NC-SA	请求全文