Lasiodiplodia: Generic revision by providing molecular markers, geographical distribution and haplotype diversity

doi:10.5943/mycosphere/14/1/14

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	Lasiodiplodia: Generic revision by providing molecular markers, geographical distribution and haplotype diversity
	Rathnayaka,A. R.; Chethana,K. W. T.; Manawasinghe,I. S.; Wijesinghe,S. N.; de Silva,N.,I; Tennakoon,D. S.; Phillips,A. J. L.; Liu,J. K.; Jones,E. B. G.; Wang,Y.; Hyde,K. D.
	2023
发表期刊	MYCOSPHERE
ISSN	2077-7019
卷号	14 期号:1 页码:1254-1339
摘要	Lasiodiplodia (Botryosphaeriaceae, Botryosphaeriales) has a global distribution and occurs on a wide range of monocotyledonous, dicotyledonous, and gymnospermous hosts. Most Lasiodiplodia species are pathogens that cause various diseases, such as stem cankers, stem and branch gummosis, shoot blight, and fruit rot. In addition, Lasiodiplodia species occur as endophytes and saprobes. This study presents one of the most reliable molecular markers for Lasiodiplodia. The combination of four loci, the internal transcribed spacer region (ITS), the partial translation elongation factor 1-a gene (tef1-a), beta-tubulin (tub2), and RNA polymerase II second largest subunit (rpb2), provided a more reliable resolution for this genus at the species level. Geographical studies showed that Lasiodiplodia species are distributed in tropical and temperate regions, but not in the polar regions. Among its species, L. theobromae has a worldwide distribution on a wide range of hosts, including economically important crops. Combining the morphology and molecular phylogeny is necessary for accurate taxonomic identification of Lasiodiplodia species and is being used in this study. Multigene phylogenetic analyses were performed based on maximum likelihood and Bayesian inference analyses using combined ITS, tef1-a, tub2, and rpb2 sequence data. In addition, the main morphological characteristics of holotype specimens of Lasiodiplodia species are provided. The current study introduced 25 new host and geographical records of saprobic Lasiodiplodia species from Taiwan province, China and Thailand. Based on the herbarium study, two Lasiodiplodia species, L. avicenniarum and L. krabiensis are synonymized under L. brasiliensis. Our analyses revelaed that Lasiodiplodia theobromae as the most common species in this genus, which occurs in a wide range of hosts in tropical and subtropical regions. The present study has led to the expansion of the taxonomic framework of Lasiodiplodia by revealing new host and geographical records. Furthermore, the haplotype networks generated using ITS, tef1-a, tub2, and rpb2 sequence data for 520 isolates of L. theobromae from 44 host families and 35 countries display that none of the L. theobromae isolates grouped according to their host family or country, suggesting that L. theobromae is a less genetically diverse, globally distributed species.
关键词	Botryosphaeriaceae Lasiodiplodia theobromae new geographical records new host recordsc 1ST REPORT GENETIC DIVERSITY SOUTH-AFRICA BOTRYOSPHAERIA-DOTHIDEA CLIMATE-CHANGE SHOOT BLIGHT PRIMER SETS TABLE GRAPE ROOT-ROT PATHOGENICITY
DOI	10.5943/mycosphere/14/1/14
WOS记录号	WOS:001069123100001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75077
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Rathnayaka,A. R.,Chethana,K. W. T.,Manawasinghe,I. S.,et al. Lasiodiplodia: Generic revision by providing molecular markers, geographical distribution and haplotype diversity[J]. MYCOSPHERE,2023,14(1):1254-1339.
APA	Rathnayaka,A. R..,Chethana,K. W. T..,Manawasinghe,I. S..,Wijesinghe,S. N..,de Silva,N.,I.,...&Hyde,K. D..(2023).Lasiodiplodia: Generic revision by providing molecular markers, geographical distribution and haplotype diversity.MYCOSPHERE,14(1),1254-1339.
MLA	Rathnayaka,A. R.,et al."Lasiodiplodia: Generic revision by providing molecular markers, geographical distribution and haplotype diversity".MYCOSPHERE 14.1(2023):1254-1339.

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