Effects of Low Nighttime Temperature on Fatty Acid Content in Developing Seeds from Brassica napus L. Based on RNA-Seq and Metabolome

doi:10.3390/plants12020325

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	Effects of Low Nighttime Temperature on Fatty Acid Content in Developing Seeds from Brassica napus L. Based on RNA-Seq and Metabolome
	Mi,Chao; Sun,Chao; Yuan,Yuting; Li,Fei; Wang,Qian; Zhu,Haiping; Hua,Shuijin; Lin,Liangbin
	2023
发表期刊	PLANTS-BASEL
ISSN	2223-7747
卷号	12 期号:2 页码:325
摘要	Brassica napus L. is a vital plant oil resource worldwide. The fatty acid biosynthesis and oil accumulation in its seeds are controlled by several genetic and environmental factors, including daytime and nighttime temperatures. We analyzed changes in oleic and erucic acid content in two double haploid (DH) lines, DH0729, a weakly temperature-sensitive line, and DH0815, a strongly temperature-sensitive line, derived from B. napus plants grown at different altitudes (1600, 1800, 2000, 2200, and 2400 m a.s.l., 28.85 degrees N, 112.35 degrees E) and nighttime temperatures (20/18, 20/16, 20/13 and 20/10 degrees C, daytime/nighttime temperature). Based on medium- and long-chain fatty acid metabolites, the total oleic acid content 35 and 43 days after flowering was significantly lower in low nighttime temperature (LNT, 20/13 degrees C) plants than in high nighttime temperature (HNT, 20/18 degrees C) plants (HNT: 58-62%; LNT: 49-54%; an average decrease of 9%), and the total erucic acid content was significantly lower in HNT than in LNT plants (HNT: 1-2%; LNT: 8-13%; an average increase of 10%). An RNA-seq analysis showed that the expression levels of SAD (LOC106366808), ECR (LOC106396280), KCS (LOC106419344), KAR (LOC106367337), HB1(LOC106430193), and DOF5 (LOC111211868) in STSL seeds increased under LNT conditions. In STSL seeds, a base mutation in the cis-acting element involved in low-temperature responsiveness (LTR), the HB1 and KCS promoter caused loss of sensitivity to low temperatures, whereas that of the KCS promoter caused increased sensitivity to low temperatures.
关键词	Brassica napus L seeds nighttime temperature fatty acid content RNA-seq metabolome cis-acting element involved in low-temperature responsiveness oleic acid erucic acid HB1 KCS TRANSCRIPTION FACTOR OIL EXPRESSION STORAGE GENES CANOLA LEAFY-COTYLEDON1 BIOSYNTHESIS ACCUMULATION REVEALS
学科领域	Plant Sciences
DOI	10.3390/plants12020325
WOS记录号	WOS:000916383600001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75044
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Mi,Chao,Sun,Chao,Yuan,Yuting,et al. Effects of Low Nighttime Temperature on Fatty Acid Content in Developing Seeds from Brassica napus L. Based on RNA-Seq and Metabolome[J]. PLANTS-BASEL,2023,12(2):325.
APA	Mi,Chao.,Sun,Chao.,Yuan,Yuting.,Li,Fei.,Wang,Qian.,...&Lin,Liangbin.(2023).Effects of Low Nighttime Temperature on Fatty Acid Content in Developing Seeds from Brassica napus L. Based on RNA-Seq and Metabolome.PLANTS-BASEL,12(2),325.
MLA	Mi,Chao,et al."Effects of Low Nighttime Temperature on Fatty Acid Content in Developing Seeds from Brassica napus L. Based on RNA-Seq and Metabolome".PLANTS-BASEL 12.2(2023):325.

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