Comparative plastome genomics and phylogenetic relationships of the genus Trollius

doi:10.3389/fpls.2023.1293091

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	Comparative plastome genomics and phylogenetic relationships of the genus Trollius
	Li,Jiaxin; Du,Yan; Xie,Lei; Jin,Xiaohua; Zhang,Zhirong; Yang,Meiqing
	2023
发表期刊	FRONTIERS IN PLANT SCIENCE
卷号	14 页码:1293091
摘要	Trollius, a genus in the Ranunculaceae family, has significant medicinal and ornamental value. It is widely distributed in China with 16 different species accepted. However, due to the lack of enough samples and information sites, the molecular phylogenetic relationships of Trollius have been unresolved till now. Here we sequenced, assembled and annotated the plastomes of 16 Trollius species to investigate their genomic characteristics, inverted repeat (IR) boundaries, sequence repeats, and hypervariable loci. In addition, the phylogenetic relationships of this genus was reconstructed based on the whole plastomes and the protein-coding sequences data-sets. The plastomes of Trollius ranged between 159,597 bp and 160,202 bp in length, and contained 113 unique genes, including 79 protein coding, 30 tRNA, and 4 rRNA. The IR boundaries were relatively conserved within the genus Trollius. 959 simple sequence repeats and 657 long sequence repeats were detected in the Trollius plastomes. We identified 12 highly polymorphic loci (Pi > 0.0115) that can be used as plastid markers in molecular identification and phylogenetic investigation of the genus. Besides, Trollius was a monophyletic group with the earliest divergence clade being Trollius lilacinus Bunge, and the remaining species were divided into two strongly-supported clades. The phylogeny in our study supported the traditional classification systems based on the color of sepal, but not the previous classification system based on the types and relative lengths of the nectaries, and distribution. The genomic resources provided in our study can be used in the taxonomy of the genus Trollius, promoting the development and utilization of this genus.
关键词	Trollius plastome comparative analysis divergent hotspots phylogenetic relationships COMPLETE CHLOROPLAST GENOME SEQUENCE RANUNCULACEAE REPEATS PLANT DNA
DOI	10.3389/fpls.2023.1293091
WOS记录号	WOS:001111730100001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74991
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Li,Jiaxin,Du,Yan,Xie,Lei,et al. Comparative plastome genomics and phylogenetic relationships of the genus Trollius[J]. FRONTIERS IN PLANT SCIENCE,2023,14:1293091.
APA	Li,Jiaxin,Du,Yan,Xie,Lei,Jin,Xiaohua,Zhang,Zhirong,&Yang,Meiqing.(2023).Comparative plastome genomics and phylogenetic relationships of the genus Trollius.FRONTIERS IN PLANT SCIENCE,14,1293091.
MLA	Li,Jiaxin,et al."Comparative plastome genomics and phylogenetic relationships of the genus Trollius".FRONTIERS IN PLANT SCIENCE 14(2023):1293091.

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