Effects of H2SO4, GA3, and cold stratification on the water content, coat composition, and dormancy release of Tilia miqueliana seeds

doi:10.3389/fpls.2023.1240028

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	Effects of H2SO4, GA3, and cold stratification on the water content, coat composition, and dormancy release of Tilia miqueliana seeds
	Wu,Yu; Huang,Wen Hui; Peng,Chen Yin; Shen,Yong Bao; Visscher,Anne M.; Pritchard,Hugh W.; Gao,Qiu; Sun,Xiao Rui; Wang,Ming Zhu; Deng,Zhiyun
	2023
发表期刊	FRONTIERS IN PLANT SCIENCE
卷号	14 页码:1240028
摘要	Introduction: Tilia miqueliana is an endemic species whose population is declining. The permeability barrier and mechanical constraint of the pericarp (seed coat) are important causes of its seed dormancy. Although there has been considerable research on this subject, questions remain regarding how the permeability barrier and mechanical constraint of the seed coat are eliminated during dormancy release and how water enters the seed. Therefore, protecting the species by improving its germination/dormancy breaking in the laboratory is urgentMethods: In this study, the changes in the cellular structure, mechanical properties, and components of the Tilia miqueliana seed coat after an H2SO4-gibberellic acid (GA(3)) treatment were analyzed during dormancy release. Various analyses (e.g., magnetic resonance imaging, scanning electron microscopy, and paraffin section detection) revealed the water gap and water channel.Results: The H2SO4 treatment eliminated the blockage at the micropyle and hilum of the seeds. Water entered the seeds through the water gap (micropyle) rather than through the hilum or seed coat, after which it dispersed along the radicle, hypocotyl, and cotyledon to the endosperm. During the cold stratification period, the cellular structure was damaged and an increasing number of holes appeared on the inner and outer surfaces of the seed coat. Vickers hardness tests showed that GA(3) decreased the seed coat hardness. Additionally, the seed coat lignin and total phenol contents continuously decreased during the cold stratification period. Notably, the Liquid chromatography-mass spectrometry (LC-MS) analysis of the seed coat detected polyethylene glycol (osmoregulator), which may have destabilized the water potential balance inside and outside the seed and increased the water content to levels required for germination, ultimately accelerating seed dormancy release.Discussion: This sophisticated and multi-level study reveals how H2SO4 and GA(3) eliminate the permeability barrier and mechanical constraints of the seed coat during dormancy release of Tilia miqueliana seeds. This will be beneficial to artificially assist the natural regeneration and population expansion of Tilia miqueliana.
关键词	H2SO4-GA3 treatment water gap and channels mechanical properties seed coat components dormancy release PHYSICAL DORMANCY STYLOSANTHES-HUMILIS GERMINATION PERMEABILITY GAP CONVOLVULACEAE ANATOMY SUGARS
DOI	10.3389/fpls.2023.1240028
WOS记录号	WOS:001114640300001
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74987
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Wu,Yu,Huang,Wen Hui,Peng,Chen Yin,et al. Effects of H2SO4, GA3, and cold stratification on the water content, coat composition, and dormancy release of Tilia miqueliana seeds[J]. FRONTIERS IN PLANT SCIENCE,2023,14:1240028.
APA	Wu,Yu.,Huang,Wen Hui.,Peng,Chen Yin.,Shen,Yong Bao.,Visscher,Anne M..,...&Deng,Zhiyun.(2023).Effects of H2SO4, GA3, and cold stratification on the water content, coat composition, and dormancy release of Tilia miqueliana seeds.FRONTIERS IN PLANT SCIENCE,14,1240028.
MLA	Wu,Yu,et al."Effects of H2SO4, GA3, and cold stratification on the water content, coat composition, and dormancy release of Tilia miqueliana seeds".FRONTIERS IN PLANT SCIENCE 14(2023):1240028.

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