Comprehensive transcriptomic profiling reveals complex molecular mechanisms in the regulation of style-length dimorphism in Guettarda speciosa (Rubiaceae), a species with anomalous distyly

doi:10.3389/fpls.2023.1116078

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	Comprehensive transcriptomic profiling reveals complex molecular mechanisms in the regulation of style-length dimorphism in Guettarda speciosa (Rubiaceae), a species with anomalous distyly
	Luo,Zhonglai; Zhao,Zhongtao; Xu,Yuanqing; Shi,Miaomiao; Tu,Tieyao; Pei,Nancai; Zhang,Dianxiang
	2023
发表期刊	FRONTIERS IN PLANT SCIENCE
卷号	14 页码:1116078
摘要	BackgroundThe evolution of heterostyly, a genetically controlled floral polymorphism, has been a hotspot of research since the 19th century. In recent years, studies on the molecular mechanism of distyly (the most common form of heterostyly) revealed an evolutionary convergence in genes for brassinosteroids (BR) degradation in different angiosperm groups. This floral polymorphism often exhibits considerable variability that some taxa have significant stylar dimorphism, but anther height differs less. This phenomenon has been termed anomalous distyly, which is usually regarded as a transitional stage in evolution. Compared to typical distyly, the genetic regulation of anomalous distyly is almost unknown, leaving a big gap in our understanding of this special floral adaptation strategy. MethodsHere we performed the first molecular-level study focusing on this floral polymorphism in Guettarda speciosa (Rubiaceae), a tropical tree with anomalous distyly. Comprehensive transcriptomic profiling was conducted to examine which genes and metabolic pathways were involved in the genetic control of style dimorphism and if they exhibit similar convergence with typical distylous species. ResultsBrassinosteroid homeostasis and plant hormone signal transduction was the most significantly enriched GO term and KEGG pathway in the comparisons between L- and S-morph styles, respectively. Interestingly, homologs of all the reported S-locus genes either showed very similar expressions between L- and S-morph styles or no hits were found in G. speciosa. BKI1, a negative regulator of brassinosteroid signaling directly repressing BRI1 signal transduction, was identified as a potential gene regulating style length, which significantly up-regulated in the styles of S-morph. DiscussionThese findings supported the hypothesis that style length in G. speciosa was regulated through a BR-related signaling network in which BKI1 may be one key gene. Our data suggested, in species with anomalous distyly, style length was regulated by gene differential expressions, instead of the hemizygous S-locus genes in typical distylous flowers such as Primula and Gelsemium, representing an intermediate stage in the evolution of distyly. Genome-level analysis and functional studies in more species with typical and anomalous distyly would further decipher this most complex marriage arrangement in angiosperms and improve our knowledge of floral evolution.
关键词	anomalous distyly brassinosteroid signaling Guettarda speciosa heterostyly molecular regulation genetic convergence PRIMULA-VULGARIS GENE-EXPRESSION ANNOTATION PATHWAY IDENTIFICATION AUXIN EVOLUTION PROTEIN GROWTH BKI1
学科领域	Plant Sciences
DOI	10.3389/fpls.2023.1116078
WOS记录号	WOS:000959028200001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74972
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Luo,Zhonglai,Zhao,Zhongtao,Xu,Yuanqing,et al. Comprehensive transcriptomic profiling reveals complex molecular mechanisms in the regulation of style-length dimorphism in Guettarda speciosa (Rubiaceae), a species with anomalous distyly[J]. FRONTIERS IN PLANT SCIENCE,2023,14:1116078.
APA	Luo,Zhonglai.,Zhao,Zhongtao.,Xu,Yuanqing.,Shi,Miaomiao.,Tu,Tieyao.,...&Zhang,Dianxiang.(2023).Comprehensive transcriptomic profiling reveals complex molecular mechanisms in the regulation of style-length dimorphism in Guettarda speciosa (Rubiaceae), a species with anomalous distyly.FRONTIERS IN PLANT SCIENCE,14,1116078.
MLA	Luo,Zhonglai,et al."Comprehensive transcriptomic profiling reveals complex molecular mechanisms in the regulation of style-length dimorphism in Guettarda speciosa (Rubiaceae), a species with anomalous distyly".FRONTIERS IN PLANT SCIENCE 14(2023):1116078.

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