Cytotoxic and pro-apoptotic effects of botanical drugs derived from the indigenous cultivated medicinal plant Paris polyphylla var. yunnanensis

doi:10.3389/fphar.2023.1100825

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	Cytotoxic and pro-apoptotic effects of botanical drugs derived from the indigenous cultivated medicinal plant Paris polyphylla var. yunnanensis
	Yan,Xiu-Xiang; Zhao,Yan-Qiang; He,Yun; Disayathanoowat,Terd; Pandith,Hataichanok; Inta,Angkhana; Yang,Li-Xin
	2023
发表期刊	FRONTIERS IN PHARMACOLOGY
ISSN	1663-9812
卷号	14 页码:1100825
摘要	Background: Cancer is one of the top two leading causes of death worldwide. Ethnobotanical research, it is one of methods, which is able to discover effective anticancer drugs based on prototype of indigenous people's historical experiences and practices. The rhizomes of Paris polyphylla var. yunnanensis (Franch.) Hand.-Mazz. have been used as botanical drugs to treat cancer by Yi, Bai, Dai, and Naxi ethnic groups in Yunnan, China, where this species is widely cultivated in a large scale in Yunnan. Materials and methods: To identify the substances of anticancer activities based on indigenous medicine knowledge, chromatography was performed to separate saponins from the rhizomes of P. polyphylla var. yunnanensis, followed by spectroscopy to determine the structure of six isolated saponins. The cytotoxicity of five extracts and six pure compounds were evaluated by MTS method. Quantitative determination of total saponins of P. polyphylla var. yunnanensis was analyzed by HPLC. Cell cycle assay, apoptosis assay, and mitochondrial membrane potential were used to evaluate the pro-apoptotic activity in vitro. Results: Five extracts and six pure saponins showed significant inhibitory cytotoxic activities of three human liver cancer cell lines (SMMC-7721, HepG2, and SK-HEP-1) and one non-small-cell lung cancer cell line (A549). The contents of Paris saponins I, II, and VII were 6.96% in the rhizomes of P. polyphylla var. yunnanensis, much higher than Chinese Pharmacopoeia standards (0.6%). Six saponins induced significant apoptosis and cell cycle arrest in three human cancer cell lines (A549, SMMC-7721, and HepG2), which was associated with the loss of mitochondrial membrane potential. Conclusion: The result of this study support that cultivated P. polyphylla var. yunnanensis could be a substitute for wild resource as an anticancer medicine based on indigenous medicine knowledge.
关键词	Paris polyphylla var yunnanensis indigenous medicine knowledge saponins cytotoxic effects pro-apoptotic effects CELL-CYCLE ARREST STEROIDAL SAPONINS HEPATOCELLULAR-CARCINOMA CHEMICAL-CONSTITUENTS ASSAY GLYCOSIDES LEAVES GROWTH STEMS SITES
学科领域	Pharmacology & Pharmacy
DOI	10.3389/fphar.2023.1100825
WOS记录号	WOS:000935013200001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74968
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Yan,Xiu-Xiang,Zhao,Yan-Qiang,He,Yun,et al. Cytotoxic and pro-apoptotic effects of botanical drugs derived from the indigenous cultivated medicinal plant Paris polyphylla var. yunnanensis[J]. FRONTIERS IN PHARMACOLOGY,2023,14:1100825.
APA	Yan,Xiu-Xiang.,Zhao,Yan-Qiang.,He,Yun.,Disayathanoowat,Terd.,Pandith,Hataichanok.,...&Yang,Li-Xin.(2023).Cytotoxic and pro-apoptotic effects of botanical drugs derived from the indigenous cultivated medicinal plant Paris polyphylla var. yunnanensis.FRONTIERS IN PHARMACOLOGY,14,1100825.
MLA	Yan,Xiu-Xiang,et al."Cytotoxic and pro-apoptotic effects of botanical drugs derived from the indigenous cultivated medicinal plant Paris polyphylla var. yunnanensis".FRONTIERS IN PHARMACOLOGY 14(2023):1100825.

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