Genetic imprints of grafting in wild iron walnut populations in southwestern China

doi:10.1186/s12870-023-04428-z

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	Genetic imprints of grafting in wild iron walnut populations in southwestern China
	Liu,Jie; Magige,Ephie A.; Fan,Peng-Zhen; Wambulwa,Moses C.; Luo,Ya-Huang; Qi,Hai-Ling; Gao,Lian-Ming; Milne,Richard I.
	2023
发表期刊	BMC PLANT BIOLOGY
卷号	23 期号:1 页码:423
摘要	Background Anthropogenic activities are causing unprecedented loss of genetic diversity in many species. However, the effects on genetic diversity from large-scale grafting onto wild plants of crop species are largely undetermined. Iron walnut (Juglans sigillata Dode) is a deciduous nut tree crop endemic to southwestern China with a long history of cultivation. Due to the rapid expansion of the walnut industry, many natural populations are now being replaced by cultivars grafted onto wild rootstocks. However, little is known about the potential genetic consequences of such action on natural populations.Results We sampled the scion and the rootstock from each of 149 grafted individuals within nine wild populations of J. sigillata from Yunnan Province which is the center of walnut diversity and cultivation in China, and examined their genetic diversity and population structure using 31 microsatellite loci. Scions had lower genetic diversity than rootstocks, and this pattern was repeated in seven of the nine examined populations. Among those seven populations, AMOVA and clustering analyses showed a clear genetic separation between all rootstocks and all scions. However, the two remaining populations, both from northern Yunnan, showed genetic similarity between scions and rootstocks, possibly indicating that wild populations here are derived from feralized local cultivars. Moreover, our data indicated probable crop-to-wild gene flow between scions and rootstocks, across all populations.Conclusions Our results indicate that large-scale grafting has been causing genetic diversity erosion and genetic structure breakdown in the wild material of J. sigillata within Yunnan. To mitigate these effects, we caution against the overuse of grafting in wild populations of iron walnut and other crop species and recommend the preservation of natural genotypes through in situ and ex situ conservation.
关键词	Conservation Genetic diversity Genetic erosion Grafting Iron walnut Juglans sigillata Microsatellite DIVERSITY DETERMINANTS CONSERVATION BIODIVERSITY SOFTWARE MARKERS FOREST YUNNAN
DOI	10.1186/s12870-023-04428-z
WOS记录号	WOS:001066167000001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74947
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Liu,Jie,Magige,Ephie A.,Fan,Peng-Zhen,et al. Genetic imprints of grafting in wild iron walnut populations in southwestern China[J]. BMC PLANT BIOLOGY,2023,23(1):423.
APA	Liu,Jie.,Magige,Ephie A..,Fan,Peng-Zhen.,Wambulwa,Moses C..,Luo,Ya-Huang.,...&Milne,Richard I..(2023).Genetic imprints of grafting in wild iron walnut populations in southwestern China.BMC PLANT BIOLOGY,23(1),423.
MLA	Liu,Jie,et al."Genetic imprints of grafting in wild iron walnut populations in southwestern China".BMC PLANT BIOLOGY 23.1(2023):423.

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