Synergistic induction of phytoalexins in Nicotiana attenuata by jasmonate and ethylene signaling mediated by NaWRKY70

doi:10.1093/jxb/erad415

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	Synergistic induction of phytoalexins in Nicotiana attenuata by jasmonate and ethylene signaling mediated by NaWRKY70
	Song, Na; Wu, Jinsong
	2024
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
卷号	75 期号:3 页码:1063-1080
摘要	Production of the phytoalexins scopoletin and scopolin is regulated by jasmonate (JA) and ethylene signaling in Nicotiana species in response to Alternaria alternata, the necrotrophic fungal pathogen that causes brown spot disease. However, how these two signaling pathways are coordinated to control this process remains unclear. In this study, we found that the levels of these two phytoalexins and transcripts of their key enzyme gene, feruloyl-CoA 6MODIFIER LETTER PRIME-hydroxylase 1 (NaF6MODIFIER LETTER PRIMEH1), were synergistically induced in Nicotiana attenuata by co-treatment with methyl jasmonate (MeJA) and ethephon. By combination of RNA sequencing and virus-induced gene silencing, we identified a WRKY transcription factor, NaWRKY70, which had a similar expression pattern to NaF6MODIFIER LETTER PRIMEH1 and was responsible for A. alternata-induced NaF6MODIFIER LETTER PRIMEH1 expression. Further evidence from stable transformed plants with RNA interference, knock out and overexpression of NaWRKY70 demonstrated that it is a key player in the synergistic induction of phytoalexins and plant resistance to A. alternata. Electrophoretic mobility shift, chromatin immunoprecipitation-quantitative PCR, and dual-luciferase assays revealed that NaWRKY70 can bind directly to the NaF6MODIFIER LETTER PRIMEH1 promoter and activate its expression. Furthermore, the key regulator of the ethylene pathway, NaEIN3-like1, can directly bind to the NaWRKY70 promoter and activate its expression. Meanwhile, NaMYC2s, important JA pathway transcription factors, also indirectly regulate the expression of NaWRKY70 and NaF6MODIFIER LETTER PRIMEH1 to control scopoletin and scopolin production. Our data reveal that these phytoalexins are synergistically induced by JA and ethylene signaling during A. alternata infection, which is largely mediated by NaWRKY70, thus providing new insights into the defense responses against A. alternata in Nicotiana species. Levels of the phytoalexins scopoletin and scopolin in Nicotiana species are synergistically induced by jasmonate and ethylene signaling during Alternaria alternata infection, largely mediated by a WRKY transcription factor, NaWRKY70.
关键词	Alternaria ethylene jasmonate Nicotiana phytoalexin scopoletin scopolin WRKY TRANSCRIPTION FACTOR ALTERNARIA-ALTERNATA GENE-EXPRESSION RESISTANCE ARABIDOPSIS SCOPOLETIN ACCUMULATION PHOSPHORYLATION BIOSYNTHESIS PERCEPTION
学科领域	Plant Sciences
DOI	10.1093/jxb/erad415
WOS记录号	WOS:001097606000001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74897
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Song, Na,Wu, Jinsong. Synergistic induction of phytoalexins in Nicotiana attenuata by jasmonate and ethylene signaling mediated by NaWRKY70[J]. JOURNAL OF EXPERIMENTAL BOTANY,2024,75(3):1063-1080.
APA	Song, Na,&Wu, Jinsong.(2024).Synergistic induction of phytoalexins in Nicotiana attenuata by jasmonate and ethylene signaling mediated by NaWRKY70.JOURNAL OF EXPERIMENTAL BOTANY,75(3),1063-1080.
MLA	Song, Na,et al."Synergistic induction of phytoalexins in Nicotiana attenuata by jasmonate and ethylene signaling mediated by NaWRKY70".JOURNAL OF EXPERIMENTAL BOTANY 75.3(2024):1063-1080.

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