Knocking down a DNA demethylase gene affects potato plant defense against a specialist insect herbivore

doi:10.1093/jxb/erad387

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	Knocking down a DNA demethylase gene affects potato plant defense against a specialist insect herbivore
	Zhang, Yadong; Zhong, Jian; Munawar, Asim; Cai, Yajie; He, Wenjing; Zhang, Yixin; Guo, Han; Gao, Yulin; Zhu, Zengrong; Zhou, Wenwu
	2024
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
卷号	75 期号:1 页码:483-499
摘要	DNA demethylase (DML) is involved in plant development and responses to biotic and abiotic stresses; however, its role in plant-herbivore interaction remains elusive. Here, we found that herbivory by the potato tuber moth, Phthorimaea operculella, rapidly induced the genome-wide DNA methylation and accumulation of DML gene transcripts in potato plants. Herbivory induction of DML transcripts was suppressed in jasmonate-deficient plants, whereas exogenous application of methyl jasmonate (MeJA) improved DML transcripts, indicating that the induction of DML transcripts by herbivory is associated with jasmonate signaling. Moreover, P. operculella larvae grew heavier on DML gene (StDML2) knockdown plants than on wild-type plants, and the decreased biosynthesis of jasmonates in the former may be responsible for this difference, since the larvae feeding on these two genotypes supplemented with MeJA showed similar growth. In addition, P. operculella adult moths preferred to oviposit on StDML2 knockdown plants than on wild-type plants, which was associated with the reduced emission of beta-caryophyllene in the former. In addition, supplementing beta-caryophyllene to these two genotypes further disrupted moths' oviposit choice preference for them. Interestingly, in StDML2 knockdown plants, hypermethylation was found at the promoter regions for the key genes StAOS and StAOC in the jasmonate biosynthetic pathway, as well as for the key gene StTPS12 in beta-caryophyllene production. Our findings suggest that knocking down StDML2 can affect herbivore defense via jasmonate signaling and defense compound production in potato plants. The DNA demethylase gene StDML2 is involved in potato plant's herbivore defense response via affecting jasmonate signaling and defense compound production in plants.
关键词	Demeter-Like DNA demethylase jasmonate Phthorimaea operculella plant herbivore defense Solanum tuberosum volatile organic compounds CRITICAL ROLES ARABIDOPSIS METHYLATION RESPONSES STRESS ROS1
学科领域	Plant Sciences
DOI	10.1093/jxb/erad387
WOS记录号	WOS:001124395900001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74896
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Zhang, Yadong,Zhong, Jian,Munawar, Asim,et al. Knocking down a DNA demethylase gene affects potato plant defense against a specialist insect herbivore[J]. JOURNAL OF EXPERIMENTAL BOTANY,2024,75(1):483-499.
APA	Zhang, Yadong.,Zhong, Jian.,Munawar, Asim.,Cai, Yajie.,He, Wenjing.,...&Zhou, Wenwu.(2024).Knocking down a DNA demethylase gene affects potato plant defense against a specialist insect herbivore.JOURNAL OF EXPERIMENTAL BOTANY,75(1),483-499.
MLA	Zhang, Yadong,et al."Knocking down a DNA demethylase gene affects potato plant defense against a specialist insect herbivore".JOURNAL OF EXPERIMENTAL BOTANY 75.1(2024):483-499.

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