NaWRKY3 is a master transcriptional regulator of the defense network against brown spot disease in wild tobacco

doi:10.1093/jxb/erad142

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	NaWRKY3 is a master transcriptional regulator of the defense network against brown spot disease in wild tobacco
	Xu,Zhen; Zhang,Shuting; Wu,Jinsong
	2023
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	1460-2431
摘要	WRKY transcription factors are involved in plant defense against pathogens. No WRKYs have been reported to be involved in resistance to tobacco brown spot disease caused by Alternaria alternata. Here, we found that NaWRKY3 plays a critical role in Nicotiana attenuata defense against A. alternata. NaWRKY3 bound and regulated many defense genes, including: lipoxygenase 3, ACC synthase 1, and ACC oxidase 1, three jasmonate- and ethylene-biosynthetic genes; feruloyl-CoA 6MODIFIER LETTER PRIME-hydroxylase 1 (NaF6MODIFIER LETTER PRIMEH1), the biosynthetic gene for the phytoalexins scopoletin and scopolin; and three A. alternata resistance genes, the long non-coding RNA L2, NADPH oxidase (NaRboh D), and berberine bridge-like (NaBBL28). Silencing L2 reduced jasmonate concentrations and NaF6MODIFIER LETTER PRIMEH1 expression. NaRboh D-silenced plants were severely impaired in reactive oxygen species production and stomatal closure responses. NaBBL28 was the first A. alternata resistance BBL identified and was involved in the hydroxylation of 17-hydroxygeranyllinalool diterpene glycosides. NaWRKY3 bound to its own promoter but repressed its expression. Thus, we demonstrated that NaWRKY3 is a fine-tuned master regulator of the defense network against A. alternata in N. attenuata by regulating several signaling pathways and defense metabolites. This is the first time such an important WRKY has been identified in Nicotiana species, providing new insights into defense against A. alternata. NaWRKY3 is a fine-tuned master regulator of the defense network against Alternaria alternatain Nicotiana attenuataby regulating jasmonate, ethylene, and reactive oxygen species signaling pathways and defense metabolites.
关键词	Alternaria BBL ethylene F6MODIFIER LETTER PRIMEH1 17-hydroxygeranyllinalool diterpene glycosides jasmonate lncRNA phytoalexin plant resistance Rboh scopoletin NICOTIANA-ATTENUATA ALTERNARIA-ALTERNATA RESISTANCE RESPONSES EXPRESSION OXIDASE PHYTOALEXIN SCOPOLETIN BURST
学科领域	Plant Sciences
DOI	10.1093/jxb/erad142
WOS记录号	WOS:000994550500001
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74895
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Xu,Zhen,Zhang,Shuting,Wu,Jinsong. NaWRKY3 is a master transcriptional regulator of the defense network against brown spot disease in wild tobacco[J]. JOURNAL OF EXPERIMENTAL BOTANY,2023.
APA	Xu,Zhen,Zhang,Shuting,&Wu,Jinsong.(2023).NaWRKY3 is a master transcriptional regulator of the defense network against brown spot disease in wild tobacco.JOURNAL OF EXPERIMENTAL BOTANY.
MLA	Xu,Zhen,et al."NaWRKY3 is a master transcriptional regulator of the defense network against brown spot disease in wild tobacco".JOURNAL OF EXPERIMENTAL BOTANY (2023).

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