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The molecular mechanism of WRINKLED1 transcription factor regulating oil accumulation in developing seeds of castor bean
Tan,Qing; Han,Bing; Haque,Mohammad Enamul; Li,Ye -Lan; Wang,Yue; Wu,Di; Wu,Shi-Bo; Liu,Ai-Zhong
2023
Source PublicationPLANT DIVERSITY
ISSN2468-2659
Volume45Issue:4Pages:469-478
AbstractThe transcription factor WRINKLED1 (WRI1), a member of AP2 gene family that contain typical AP2 domains, has been considered as a master regulator regulating oil biosynthesis in oilseeds. However, the regulatory mechanism of RcWRI1 in regulating oil accumulation during seed development has not been clearly addressed. Castor bean (Ricinus communis) is one of the most important non-edible oil crops and its seed oils are rich in hydroxy fatty acids, widely applied in industry. In this study, based on castor bean reference genome, three RcWRIs genes (RcWRI1, RcWRI2 and RcWRI3) were identified and the expressed association of RcWRI1 with oil accumulation were determined. Heterologous transformation of RcWRI1 significantly increased oil content in tobacco leaf, confirming that RcWRI1 activate lipid biosynthesis pathway. Using DNA Affinity Purification sequencing (DAP-seq) technology, we confirmed RcWRI1 binding with Transcription Start Site of genes and identified 7961 WRI1-binding candidate genes. Functionally, these identified genes were mainly involved in diverse metabolism pathways (including lipid biosynthesis). Three cis-elements AW-box ([CnTnG](n)7[CG]) and AW-boxes like ([GnAnC](n)6[GC]/ [GnAnC](n)7[G]) bound with RcWRI1 were identified. Co-expression network analysis of RcWRI1 further found that RcWRI1 might be widely involved in biosynthesis of storage materials during seed devel-opment. In particular, yeast one hybrid experiments found that both AP2 domains within RcWRI1 were required in binding targeted genes. These results not only provide new evidence to understand the regulatory mechanism of RcWRI1 in regulation of oil accumulation during castor bean seed develop-ment, but also give candidate gene resource for subsequent genetic improvement toward increasing oil content in oilseed crops. Copyright & COPY; 2022 Kunming Institute of Botany, Chinese Academy of Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY -NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
KeywordCastor bean WRI transcription factor Oil accumulation Developing seeds Lipid gene FATTY-ACID BIOSYNTHESIS KEY REGULATOR ARABIDOPSIS DOMAIN EXPRESSION INDUSTRIAL PATHWAYS STORAGE GENES LIGHT
DOI10.1016/j.pld.2022.09.003
WOS IDWOS:001052651400001
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Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/74855
Collection中国科学院昆明植物研究所
Recommended Citation
GB/T 7714
Tan,Qing,Han,Bing,Haque,Mohammad Enamul,et al. The molecular mechanism of WRINKLED1 transcription factor regulating oil accumulation in developing seeds of castor bean[J]. PLANT DIVERSITY,2023,45(4):469-478.
APA Tan,Qing.,Han,Bing.,Haque,Mohammad Enamul.,Li,Ye -Lan.,Wang,Yue.,...&Liu,Ai-Zhong.(2023).The molecular mechanism of WRINKLED1 transcription factor regulating oil accumulation in developing seeds of castor bean.PLANT DIVERSITY,45(4),469-478.
MLA Tan,Qing,et al."The molecular mechanism of WRINKLED1 transcription factor regulating oil accumulation in developing seeds of castor bean".PLANT DIVERSITY 45.4(2023):469-478.
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