RAD-sequencing improves the genetic characterization of a threatened tree peony (Paeonia ludlowii) endemic to China: Implications for conservation

doi:10.1016/j.pld.2022.07.002

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	RAD-sequencing improves the genetic characterization of a threatened tree peony (Paeonia ludlowii) endemic to China: Implications for conservation
	Zhao,Yu-Juan; Yin,Gen-Shen; Gong,Xun
	2023
发表期刊	PLANT DIVERSITY
ISSN	2468-2659
卷号	45 期号:5 页码:513-522
摘要	Compared with traditional genetic markers, genomic approaches have proved valuable to the conservation of endangered species. Paeonia ludlowii having rarely and pure yellow flowers, is one of the world's most famous tree peonies. However, only several wild populations remain in the Yarlung Zangbo Valley (Nyingchi and Shannan regions, Xizang) in China due to increasing anthropogenic impact on the natural habitats. We used genome-wide single nucleotide polymorphisms to elucidate the spatial pattern of genetic variation, population structure and demographic history of P. ludlowii from the fragmented region comprising the entire range of this species, aiming to provide a basis for conserving the genetic resources of this species. Unlike genetic uniformity among populations revealed in previous studies, we found low but varied levels of intra-population genetic diversity, in which lower genetic diversity was detected in the population in Shannan region compared to those in Nyingzhi region. These spatial patterns may be likely associated with different population sizes caused by micro-environment differences in these two regions. Additionally, low genetic differentiation among populations (Fst 1/4 0.0037) were detected at the species level. This line of evidence, combined with the result of significant genetic differentiation between the two closest populations and lack of isolation by distance, suggested that shared ancestry among now remnant populations rather than contemporary genetic connectivity resulted in subtle population structure. Demographic inference suggested that P. ludlowii probably experienced a temporal history of sharp population decline during the period of Last Glacial Maximum, and a subsequent bottleneck event resulting from prehistoric human activities on the Qinghai-Tibet Plateau. All these events, together with current habitat fragment and excavation might contribute to the endangered status of P. ludlowii. Our study improved the genetic characterization of the endangered tree peony (P. ludlowii) in China, and these genetic inferences should be considered when making different in situ and ex situ conservation actions for P. ludlowii in this evolutionary hotspot region.Copyright (c) 2022 Kunming Institute of Botany, Chinese Academy of Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
关键词	Conservation Fragmentation Genetic structure Genetic variation Paeonia RAD-sequencing HABITAT FRAGMENTATION POPULATION-STRUCTURE GENOMICS CONSEQUENCES DIVERSITY SOFTWARE FLOW CHALLENGES INFERENCE DISTANCE
DOI	10.1016/j.pld.2022.07.002
WOS记录号	WOS:001090171700001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74854
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Zhao,Yu-Juan,Yin,Gen-Shen,Gong,Xun. RAD-sequencing improves the genetic characterization of a threatened tree peony (Paeonia ludlowii) endemic to China: Implications for conservation[J]. PLANT DIVERSITY,2023,45(5):513-522.
APA	Zhao,Yu-Juan,Yin,Gen-Shen,&Gong,Xun.(2023).RAD-sequencing improves the genetic characterization of a threatened tree peony (Paeonia ludlowii) endemic to China: Implications for conservation.PLANT DIVERSITY,45(5),513-522.
MLA	Zhao,Yu-Juan,et al."RAD-sequencing improves the genetic characterization of a threatened tree peony (Paeonia ludlowii) endemic to China: Implications for conservation".PLANT DIVERSITY 45.5(2023):513-522.

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