Moringa oleifera seed ethanol extract and its active component kaempferol potentiate pentobarbital-induced sleeping behaviours in mice via a GABAergic mechanism

doi:10.1080/13880209.2022.2056207

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	Moringa oleifera seed ethanol extract and its active component kaempferol potentiate pentobarbital-induced sleeping behaviours in mice via a GABAergic mechanism
	Liu, Wei-Liang; Wu, Bai-Fen; Shang, Jian-Hua; Wang, Xue-Feng; Zhao, Yun-Li; Huang, Ai-Xiang
	2022
发表期刊	PHARMACEUTICAL BIOLOGY
卷号	60 期号:1 页码:810-824
摘要	Context Moringa oleifera Lam. (Moringaceae) (MO) is an important food plant that has high nutritional and medical value. However, there is limited information on whether its seeds can improve sleep. Objective This study investigated the effects of MO seed ethanol extracts (EEMOS) on sleep activity improvement and examined the underlying mechanisms. Materials and methods Male ICR mice were placed into six groups (n = 12) and treated as follows: Control (sodium carboxymethyl cellulose, 20 mL/kg), estazolam tablets (2 mg/kg), EEMOS (1, 2 g/kg) and kaempferol (1, 2 mg/kg). These samples were successively given intragastric for 14 d. Locomotor activity assay, pentobarbital-induced sleeping and pentetrazol-induced seizures tests were utilized to examine the sedative-hypnotic effects (SHE) of EEMOS. Results Compared with the control group, the results revealed that EEMOS (2 g/kg) and KA (2 mg/kg) possessed good SHE and could significantly elevate the levels of gamma-aminobutyric acid and reduce the levels of glutamic acid in the mouse hypothalamus (p < 0.05). Moreover, SHE was blocked by picrotoxin, flumazenil and bicuculline (p < 0.05). EEMOS (2 g/kg) and KA (2 mg/kg) significantly upregulated the protein expression levels of glutamic acid decarboxylase-65 (GAD(65)) and alpha(1)-subunit of GABA(A) receptors in the hypothalamus of mice (p < 0.05), not affecting glutamic acid decarboxylase-67 (GAD(67)) and gamma(2)-subunit expression levels (p > 0.05). Additionally, they cause a significant increase in Cl- influx in human cerebellar granule cells at a concentration of 8 mu g/mL (p < 0.05). Discussion and conclusions These findings demonstrated that EEMOS could improve sleep by regulating GABA(A)-ergic systems, and encourage further clinical trials to treat insomnia.
关键词	Sedative-hypnotic Cl- influx GABA(A) receptors GABA(A)-ergic systems BLOOD-BRAIN-BARRIER GABA(A) RECEPTORS INSOMNIA MODULATION PHYSIOLOGY FLAVONOIDS TOXICITY ANXIETY NEURONS DISEASE
DOI	10.1080/13880209.2022.2056207
收录类别	SCI
WOS记录号	WOS:000797801700001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74780
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Liu, Wei-Liang,Wu, Bai-Fen,Shang, Jian-Hua,et al. Moringa oleifera seed ethanol extract and its active component kaempferol potentiate pentobarbital-induced sleeping behaviours in mice via a GABAergic mechanism[J]. PHARMACEUTICAL BIOLOGY,2022,60(1):810-824.
APA	Liu, Wei-Liang,Wu, Bai-Fen,Shang, Jian-Hua,Wang, Xue-Feng,Zhao, Yun-Li,&Huang, Ai-Xiang.(2022).Moringa oleifera seed ethanol extract and its active component kaempferol potentiate pentobarbital-induced sleeping behaviours in mice via a GABAergic mechanism.PHARMACEUTICAL BIOLOGY,60(1),810-824.
MLA	Liu, Wei-Liang,et al."Moringa oleifera seed ethanol extract and its active component kaempferol potentiate pentobarbital-induced sleeping behaviours in mice via a GABAergic mechanism".PHARMACEUTICAL BIOLOGY 60.1(2022):810-824.

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