Branched Chondroitin Sulfate Oligosaccharides Derived from the Sea Cucumber Acaudina molpadioides Stimulate Neurite Outgrowth

doi:10.3390/md20100653

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	Branched Chondroitin Sulfate Oligosaccharides Derived from the Sea Cucumber Acaudina molpadioides Stimulate Neurite Outgrowth
	Wang, Weili; Mao, Hui; Li, Sujuan; Zhang, Longlong; Yang, Lian; Yin, Ronghua; Zhao, Jinhua
	2022
发表期刊	MARINE DRUGS
卷号	20 期号:10 页码:653
摘要	Fucosylated chondroitin sulfate (FCS) from the sea cucumber Acaudina molpadioides (FCSAm) is the first one that was reported to be branched by disaccharide GalNAc-(alpha 1,2)-Fuc(3S4S) (15%) and sulfated Fuc (85%). Here, four size-homogenous fractions, and seven oligosaccharides, were separated from its beta-eliminative depolymerized products. Detailed NMR spectroscopic and MS analyses revealed the oligomers as hexa-, hepta-, octa-, and nonasaccharide, which further confirmed the precise structure of native FCSAm: it was composed of the CS-E-like backbone with a full content of sulfation at O-4 and O-6 of GalNAc in the disaccharide repeating unit, and the branches consisting of sulfated fucose (Fuc(4S) and Fuc(2S4S)) and heterodisaccharide [GalNAc-(alpha 1,2)-Fuc(3S4S)]. Pharmacologically, FCSAm and its depolymerized derivatives, including fractions and oligosaccharides, showed potent neurite outgrowth-promoting activity in a chain length-dependent manner. A comparison of analyses among oligosaccharides revealed that the sulfate pattern of the Fuc branches, instead of the heterodisaccharide, could affect the promotion intensity. Fuc(2S4S) and the saccharide length endowed the neurite outgrowth stimulation activity most.
关键词	fucosylated chondroitin sulfate sea cucumber disaccharide branch oligosaccharide neurite outgrowth stimulation HIPPOCAMPAL-NEURONS PROMOTING ACTIVITY GLYCOSAMINOGLYCAN DISACCHARIDE INHIBITOR
DOI	10.3390/md20100653
收录类别	SCI
WOS记录号	WOS:000873081700001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74740
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Wang, Weili,Mao, Hui,Li, Sujuan,et al. Branched Chondroitin Sulfate Oligosaccharides Derived from the Sea Cucumber Acaudina molpadioides Stimulate Neurite Outgrowth[J]. MARINE DRUGS,2022,20(10):653.
APA	Wang, Weili.,Mao, Hui.,Li, Sujuan.,Zhang, Longlong.,Yang, Lian.,...&Zhao, Jinhua.(2022).Branched Chondroitin Sulfate Oligosaccharides Derived from the Sea Cucumber Acaudina molpadioides Stimulate Neurite Outgrowth.MARINE DRUGS,20(10),653.
MLA	Wang, Weili,et al."Branched Chondroitin Sulfate Oligosaccharides Derived from the Sea Cucumber Acaudina molpadioides Stimulate Neurite Outgrowth".MARINE DRUGS 20.10(2022):653.

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