Cold Response Transcriptome Analysis of the Alternative Splicing Events Induced by the Cold Stress in D. catenatum

doi:10.3390/ijms23020981

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	Cold Response Transcriptome Analysis of the Alternative Splicing Events Induced by the Cold Stress in D. catenatum
	Zheng, Yan; Luo, Landi; Chen, Qian; Yang, Danni; Gong, Yuqiang; Yang, Ya; Qin, Xiangshi; Wang, Yuhua; Kong, Xiangxiang; Yang, Yongping
	2022
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	23 期号:2 页码:981
摘要	Dendrobium catenatum Lindl is a valuable medicinal herb and gardening plant due to its ornamental value and special medical value. Low temperature is a major bottleneck restricting D. catenatum expansion towards the north, which influences the quality and yield of D. catenatum. In this study, we analysed the cold response of D. catenatum by RNA-Seq. A total of 4302 differentially expressed genes were detected under cold stress, which were mainly linked to protein kinase activity, membrane transport and the glycan biosynthesis and metabolism pathway. We also identified 4005 differential alternative events in 2319 genes significantly regulated by cold stress. Exon skipping and intron retention were the most common alternative splicing isoforms. Numerous genes were identified that differentially modulated under cold stress, including cold-induced transcription factors and splicing factors mediated by AS (alternative splicing). GO enrichment analysis found that differentially alternatively spliced genes without differential expression levels were related to RNA/mRNA processing and spliceosomes. DAS (differentially alternative splicing) genes with different expression levels were mainly enriched in protein kinase activity, plasma membrane and cellular response to stimulus. We further identified and cloned DcCBP20 in D. catenatum; we found that DcCBP20 promotes the generation of alternative splicing variants in cold-induced genes under cold stress via genetic experiments and RT-PCR. Taken together, our results identify the main cold-response pathways and alternative splicing events in D. catenatum responding to cold treatment and that DcCBP20 of D. catenatum get involved in regulating the AS and gene expression of cold-induced genes during this process. Our study will contribute to understanding the role of AS genes in regulating the cold stress response in D. catenatum.
关键词	Dendrobium catenatum Lindl alternative splicing cold stress DcCBP20 CAP-BINDING COMPLEX FREEZING TOLERANCE LOW-TEMPERATURE ARABIDOPSIS EXPRESSION PROTEINS GENES ACCLIMATION LANDSCAPE REVEALS
DOI	10.3390/ijms23020981
收录类别	SCI
WOS记录号	WOS:000749793200001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74715
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Zheng, Yan,Luo, Landi,Chen, Qian,et al. Cold Response Transcriptome Analysis of the Alternative Splicing Events Induced by the Cold Stress in D. catenatum[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(2):981.
APA	Zheng, Yan.,Luo, Landi.,Chen, Qian.,Yang, Danni.,Gong, Yuqiang.,...&Yang, Yongping.(2022).Cold Response Transcriptome Analysis of the Alternative Splicing Events Induced by the Cold Stress in D. catenatum.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(2),981.
MLA	Zheng, Yan,et al."Cold Response Transcriptome Analysis of the Alternative Splicing Events Induced by the Cold Stress in D. catenatum".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.2(2022):981.

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