Epidemic Identification of Fungal Diseases in Morchella Cultivation across China

doi:10.3390/jof8101107

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	Epidemic Identification of Fungal Diseases in Morchella Cultivation across China
	Shi, Xiaofei; Liu, Dong; He, Xinhua; Liu, Wei; Yu, Fuqiang
	2022
发表期刊	JOURNAL OF FUNGI
卷号	8 期号:10 页码:1107
摘要	True morels (Morchella, Pezizales) are world-renowned edible mushrooms (ascocarps) that are widely demanded in international markets. Morchella has been successfully artificially cultivated since 2012 in China and is rapidly becoming a new edible mushroom industry occupying up to 16,466 hectares in the 2021-2022 season. However, nearly 25% of the total cultivation area has annually suffered from fungal diseases. While a variety of morel pathogenic fungi have been reported their epidemic characteristics are unknown, particularly in regional or national scales. In this paper, ITS amplicon sequencing and microscopic examination were concurrently performed on the morel ascocarp lesions from 32 sites in 18 provinces across China. Results showed that Diploospora longispora (75.48%), Clonostachys solani (5.04%), Mortierella gamsii (0.83%), Mortierella amoeboidea (0.37%) and Penicillium kongii (0.15%) were the putative pathogenic fungi. The long, oval, septate conidia of D. longispora was observed on all ascocarps. Oval asexual spores and sporogenic structures, such as those of Clonostachys, were also detected in C. solani infected samples with high ITS read abundance. Seven isolates of D. longispora were isolated from seven selected ascocarps lesions. The microscopic characteristics of pure cultures of these isolates were consistent with the morphological characteristics of ascocarps lesions. Diploospora longispora had the highest amplification abundance in 93.75% of the samples, while C. solani had the highest amplification abundance in six biological samples (6.25%) of the remaining two sampling sites. The results demonstrate that D. longispora is a major culprit of morel fungal diseases. Other low-abundance non-host fungi appear to be saprophytic fungi infecting after D. longispora. This study provides data supporting the morphological and molecular identification and prevention of fungal diseases of morel ascocarps.
关键词	Clonostachys solani Diploospora longispora ITS amplicon sequencing fungal disease morels cultivation SPECIES COMPLEX
DOI	10.3390/jof8101107
收录类别	SCI
WOS记录号	WOS:000873005000001
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74705
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Shi, Xiaofei,Liu, Dong,He, Xinhua,et al. Epidemic Identification of Fungal Diseases in Morchella Cultivation across China[J]. JOURNAL OF FUNGI,2022,8(10):1107.
APA	Shi, Xiaofei,Liu, Dong,He, Xinhua,Liu, Wei,&Yu, Fuqiang.(2022).Epidemic Identification of Fungal Diseases in Morchella Cultivation across China.JOURNAL OF FUNGI,8(10),1107.
MLA	Shi, Xiaofei,et al."Epidemic Identification of Fungal Diseases in Morchella Cultivation across China".JOURNAL OF FUNGI 8.10(2022):1107.

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