Temporal regulation of alternative splicing events in rice memory under drought stress

doi:10.1016/j.pld.2020.11.0042468-2659

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	Temporal regulation of alternative splicing events in rice memory under drought stress
	Yang, Hong; Li, Ping; Jin, Guihua; Gui, Daping; Liu, Li; Zhang, Chengjun
	2022
发表期刊	PLANT DIVERSITY
卷号	44 期号:1 页码:116-125
摘要	Plant adaptation to drought stress is essential for plant survival and crop yield. Recently, harnessing drought memory, which is induced by repeated stress and recovery cycles, was suggested as a means to improve drought resistance at the transcriptional level. However, the genetic mechanism underlying drought memory is unclear. Here, we carried out a quantitative analysis of alternative splicing (AS) events in rice memory under drought stress, generating 12 transcriptome datasets. Notably, we identified exon skipping (ES) as the predominant AS type (>80%) in differential alternative splicing (DAS) in response to drought stress. Applying our analysis pipeline to investigate DAS events following drought stress in six other plant species revealed variable ES frequencies ranging from 9.94% to 60.70% depending on the species, suggesting that the relative frequency of DAS types in plants is likely to be species specific. The dinucleotide sequence at AS splice sites in rice following drought stress was preferentially GC-AG and AT-AC. Since U12-type splicing uses the AT-AC site, this suggests that drought stress may increase U12-type splicing, and thus increase ES frequency. We hypothesize that multiple isoforms derived from exon skipping may be induced by drought stress in rice. We also identified 20 transcription factors and three highly connected hub genes with potential roles in drought memory that may be good targets for plant breeding. Copyright (c) 2020 Kunming Institute of Botany, Chinese Academy of Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
关键词	Alternative splicing Drought memory Exon skipping TRANSCRIPTION FACTOR FAMILY GENOME-WIDE ANALYSIS RNA-SEQ GENE IDENTIFICATION SPLICEOSOME COMPLEXITY TOLERANCE LANDSCAPE PROTEIN
DOI	10.1016/j.pld.2020.11.0042468-2659
收录类别	SCI
WOS记录号	WOS:000761555200001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74678
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Yang, Hong,Li, Ping,Jin, Guihua,et al. Temporal regulation of alternative splicing events in rice memory under drought stress[J]. PLANT DIVERSITY,2022,44(1):116-125.
APA	Yang, Hong,Li, Ping,Jin, Guihua,Gui, Daping,Liu, Li,&Zhang, Chengjun.(2022).Temporal regulation of alternative splicing events in rice memory under drought stress.PLANT DIVERSITY,44(1),116-125.
MLA	Yang, Hong,et al."Temporal regulation of alternative splicing events in rice memory under drought stress".PLANT DIVERSITY 44.1(2022):116-125.

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