Detailed Structural Analysis of the Immunoregulatory Polysaccharides from the Mycobacterium Bovis BCG

doi:10.3390/molecules27175691

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	Detailed Structural Analysis of the Immunoregulatory Polysaccharides from the Mycobacterium Bovis BCG
	Luo, Lan; Song, Xuemei; Chang, Xiao; Huang, Sheng; Zhou, Yunxi; Yang, Shengmei; Zhu, Yan; Zhang, Lanyan; Wu, Yongsheng; Zhang, Junyin; Zhou, Zhipeng; Wu, Mingyi
	2022
发表期刊	MOLECULES
卷号	27 期号:17 页码:5691
摘要	Bacillus Calmette-Guerin polysaccharide and nucleic acid (BCG-PSN), extracted from Mycobacterium bovis, is an immunoregulatory medicine commonly used in clinic. However, the structural characteristics and potential pharmacological efficacy of the polysaccharides from BCG-PSN remain unclear. Herein, two polysaccharides (BCG-1 and BCG-2) were purified and their structures were characterized. Monosaccharide composition analysis combined with methylation analysis and NMR data indicated that BCG-1 and BCG-2 were an alpha-D-(1 -> 4)-mannan with (1 -> 2)-linked branches, and an alpha-D-(1 -> 4)-glucan with (1 -> 6)-linked branches, respectively. Herein, the mannan from BCG-PSN was first reported. Bioactivity assays showed that BCG-1 and BCG-2 dose-dependently and potently increased the production of inflammatory mediators (NO, TNF-alpha, IL-6, IL-1 beta, and IL-10), as well as their mRNA expressions in RAW264.7 cells; both have similar or stronger effects compared with BCG-PSN injection. These data suggest that BCG-1 and BCG-2 are very likely the active ingredients of BCG-PSN.
关键词	active ingredients polysaccharide purification glucan mannan immunomodulator BACILLUS-CALMETTE-GUERIN (BCG)-INDUCED MACROPHAGE CYTOTOXICITY IMMUNOMODULATORY ACTIVITY MALDI-TOF INTERLEUKIN-10 ACTIVATION IMMUNITY GLUCANS SULFATE MANNAN
DOI	10.3390/molecules27175691
收录类别	SCI
WOS记录号	WOS:000851767500001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74673
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Luo, Lan,Song, Xuemei,Chang, Xiao,et al. Detailed Structural Analysis of the Immunoregulatory Polysaccharides from the Mycobacterium Bovis BCG[J]. MOLECULES,2022,27(17):5691.
APA	Luo, Lan.,Song, Xuemei.,Chang, Xiao.,Huang, Sheng.,Zhou, Yunxi.,...&Wu, Mingyi.(2022).Detailed Structural Analysis of the Immunoregulatory Polysaccharides from the Mycobacterium Bovis BCG.MOLECULES,27(17),5691.
MLA	Luo, Lan,et al."Detailed Structural Analysis of the Immunoregulatory Polysaccharides from the Mycobacterium Bovis BCG".MOLECULES 27.17(2022):5691.

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