药用植物天名精化学成分及其生物活性研究

	药用植物天名精化学成分及其生物活性研究; Study on the Compositions from Carpesium abrotanoides L. and Their Bioactivities
	杨宝嘉
导师	李顺林
摘要	The genus Carpesium (Asteraceae) is a traditional herbal medicine and widely used to treat various ailments in Southeast Asian countries. Carpesium abrotanoides L. has long history of medicine in this genus. That covers diverse and complex secondary metabolites with the functions of eliminating heat, detoxification and hemostasis. However, the active ingredients and mechanism of action are still unclear. Natural products have provided unlimited resources for drug discovery because of their inherent chemical diversity. Therefore, we systematically carried out phytochemical research on C. abrotanoides and explored the bioactivity of their isolates. The thesis consists of three chapters. In chapter one, we discussed an investigation of chemical constituents from C. abrotanoides. A total of forty-one known compounds were isolated from the methanol extract of this plant using various column chromatography, besides of twenty-four sesquiterpenes, six menthane-type monoterpenes and eleven other types of compounds. Their structures were elucidated by analysis of their NMR and MS data as well as by comparison with the literatures. In chapter two, we studied on the biological activity of the ingredients isolated from C. abrotanoides. We have evaluated the activity of the isolated terpenoids from two biological functions. One is pharmacology, which involves anti-tumor, lysosomal biogenesis, and anti-inflammatory. The other is eco-friendly pesticides, including insecticidal effects, plant virus resistance, and phytotoxicity. All selected compounds were screened for their cytotoxic activity. Specially, compound 4 showed the strongest activity against five human cancer cell lines, including A549, HepG2, HCT116, MDA-MB-231, and CNE2. Further investigation revealed that compound 4 effectively induced G2/M cell cycle arrest and reactive oxygen species (ROS) accumulation in a dose-dependent manner, which further led to apoptosis in cancer cells. Interestingly, compounds 4 and 6 could also induce cytoprotective autophagy and regulate the expression of lysosome-related genes, including LAMP1, CTSB, CTSA, ATP6V0E1 and ARSB, and promoting lysosomal biogenesis. In addition, compounds 4-5, 12, 14 and 19 showed significant anti-inflammatory activity, with an inhibition rate higher than 90%. Another concern is that some sesquiterpene lactones have revealed their effective potential value in the innovation of plant pesticides. Both of compounds 2 and 19 displayed strong effects in the prevention of Tomato Spotted Wilt Virus (TSWV) and Tobacco Mosaic Virus (TMV), and their inhibitory effects were better than Ningnanmycin. And the high content of compound 6 could also significantly inhibit seed germination and root elongation. Therefore, we have confirmed that the sesquiterpene lactones have diverse and significant biological activities. Surprisingly, it is the first report that the ingredients of the genus Carpesium have significant new biological activities of promoting ly
	2021-05
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74617
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	杨宝嘉. 药用植物天名精化学成分及其生物活性研究, Study on the Compositions from Carpesium abrotanoides L. and Their Bioactivities[D],2021.

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