桃蚜~菟丝子~黄瓜互作系统中 mRNA 的转运研究

	桃蚜~菟丝子~黄瓜互作系统中 mRNA 的转运研究; Study of mRNA transfer in the green peach aphid-dodder-cucumber interaction system
	卞金鸽
导师	吴建强
摘要	While most plants in nature live autotrophically through photosynthesis, there is a group of plants that partly or completely depend on other plants for water and nutrients, which are called parasitic plants. Currently, about 4,530 species of parasitic plants are found, accounting for 1.2% of flowering plants (369,000). Parasitic plants establish vascular connections with their hosts through a special organ, the haustorium, through which the parasitic plants can obtain inorganic salts, water, nutrients, etc., which are necessary to sustain life. In addition, secondary metabolites, mRNAs, proteins and even viruses from the host can be transferred to the parasitic plant. Dodder is an annual stem parasitic plant of the genus Cuscuta in the Convolvulaceae family. Dodder has no roots or leaves and must parasitize a host after germination. Dodder invades the host's stem through haustoria and connects with the host's vascular tissue to form a channel for material exchange. Dodder can parasitize multiple hosts at the same time, forming a plant cluster. There is a large amount of exchange of substances with the members in this plant cluster, such as the systemic signals for salt tolerance, insect resistance and nitrogen stress. Transcriptomic studies have shown that thousands of mRNAs are already exchanged between dodder and hosts in the absence of any biotic or abiotic stresses. The transferred mRNAs could be degraded or accumulated in the host or could express proteins in the host for biological functions. Aphids are one of the few piercing-sucking insects that can feed on dodder, and the green peach aphid (GPA, Myzus persicae), a generalist aphid, is a major threat to agricultural production. During feeding, the GPAs not only feed on the host sap through their mouthparts, but also transport a variety of proteins, mRNAs, and other macromolecules into the plant. The GPAs can feed on and rapidly reproduce on dodders. It has been found that feeding on dodders by GPAs enhances the host soybean's ability to resist aphid feeding, suggesting that the systemic signal of insect resistance can be transferred from dodders to soybeans, thereby enhancing the host soybean's resistance to insects. To date, there have been many studies on the transport of substances and signals between dodder and hosts, especially the transport of mRNAs. Studies on mRNAs translocation have been more focused on the relationship between dodder and the host, whether there is trafficking of mRNAs among aphids, dodders, and host plants and if aphid feeding affects the mRNA transfer between dodders and hosts are still unclear.In this thesis, based on the establishment of a dodder-cucumber hydroponic parasitic system, the GPAs were used to feed on dodder, in order to investigate the mRNA trafficking between GPA-dodder-cucumber leaves and roots. The inter-kingdom mRNA transfer in the animal-parasitic plant-plant system was resolved for the first time by using transcriptomic sequencing.
	2021-05
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74591
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	卞金鸽. 桃蚜~菟丝子~黄瓜互作系统中 mRNA 的转运研究, Study of mRNA transfer in the green peach aphid-dodder-cucumber interaction system[D],2021.

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