三七皂苷炮制品改善小鼠脂代谢的作用及机制研究

	三七皂苷炮制品改善小鼠脂代谢的作用及机制研究; The Function and Mechanism Study of Steamed Saponins of Panax notoginseng (Burk.) F. H. Chen in Lipid Metabolism in Mice
	王倩
导师	熊文勇
摘要	In recent years, with the improvement of people's living standard and the change of diet structure, the morbidity of obesity increases year by year. Obesity can lead to metabolic diseases such as diabetes, cardiovascular disease and insulin resistance. At present, natural products provide rich resources for drug screening and discovery. It is still an urgent problem to develop a safe and effective lipid-lowering drug with low toxic and side effects from natural products. Saponins of Panax notoginseng (Burk.) F. H. Chen has been classified as a type of composition in functional foods for numerous diseases. However, its mild effects and other characteristics limited clinical applications in diseases. Based on the comprehensive and optimal utilization of P. notoginseng resources, in order to further explore its potential medicinal value, this paper mainly studied the effect and mechanism of the processed P. notoginseng saponins on improving lipid metabolism in mice. Inspired by “nine-steaming and nine-processing” of P. notoginseng in traditional Chinese medicine, we developed a “steaming”-mimic protocol which significantly changed the composition of saponins of P. notoginseng from the original: R1, Rg1, Re, Rb1 and Rd (Raw-PNS) to the products after steaming: 20S/R-Rh1, Rk3, Rh4, 20S/R-Rg3, Rk1 and Rg5 (N-PNS). The content of total saponins in P. notoginseng with different processing times was determined and the corresponding component peaks were specified. The lipid lowering activities of P. notoginseng saponins with different processing times were studied, and the pharmacological activities of P. notoginseng saponins in improving obesity and hyperlipidemia were evaluated. Surprisingly, the N-PNS demonstrated promising activities in improving hyperlipidemia and reducing body weight and weight of WAT, and the inhibition of adipogenesis in obese mice. In accordance with the results in vivo, N-PNS remarkably blunted adipogenesis at the early stage of differentiation dose-dependently in vitro. Moreover, we demonstrated that the activity may be involved AMPK signaling pathway by promoting phosphorylation of AMPKT172 and down-regulating its downstream factors: SREBP-1c, SCD1 and FAS. Taken together, the steaming-induced eight compositions of saponins showed a very promising function in improving hyperlipidemia and obesity both in vivo and in vitro, providing fundamental evidences for future study and application in treatment of hyperlipidemia, obesity and other lipids-related metabolic syndromes.
	2021-05
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74580
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	王倩. 三七皂苷炮制品改善小鼠脂代谢的作用及机制研究, The Function and Mechanism Study of Steamed Saponins of Panax notoginseng (Burk.) F. H. Chen in Lipid Metabolism in Mice[D],2021.

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