苦瓜 McOSC 和 McP450 的功能鉴定及在三萜 生物合成中的初步应用

	苦瓜 McOSC 和 McP450 的功能鉴定及在三萜生物合成中的初步应用; Functional identification of McOSC and McP450 from bitter gourd（Momordica charantia L.）and its preliminary application in triterpenoid biosynthesis
	韩雨桐
导师	杨雪飞
摘要	Triterpenoids as a research focus of natural products have anti-tumor, anti-inflammatory, anti-viral, anti-hypertensive, lipid-regulating, hypoglycemic, anti-AIDS and other activities. It was found that compared with monocotyledonous plants, triterpenoids were more widely distributed and varied in dicotyledonous plants, especially in Cucurbitaceae, Acanthaceae, Leguminosae and Aesculaceae. Momordica charantia L. is an annual climbing herb of Cucurbitaceae, also known as bitter gourd and bitter melon. As a common vegetable, its fruit contains multiple triterpenoids with functions of hypoglycemia, anti-obesity, anti-diabetes, anti-cancer and anti-gluconeogenesis. Due to the low efficiency of separation, extraction or chemical synthesis of triterpenoids in bitter gourd, their applications have been limited. Therefore, in recent years, studies on triterpenoids in bitter gourd have shifted to biosynthesis pathway. In this study, the transcriptome and triterpenoid metabolites at different growth stages of bitter gourd were analyzed; the expression patterns of triterpenoid synthesis candidate gene were explored. The McOSC and McP450 that formed gene clusters adjacent to each other were screened by chromosomal localization, and their functions in the synthesis of triterpenoids in bitter gourd were analyzed by exogenous gene expression test, which laid a foundation for the subsequent heterologous expression of triterpenoids in bitter gourd. The main results are as follows: （1）Transcriptome and metabolome of bitter gourd during development （2）Identification and expression pattern analysis of triterpenoid synthetic genes in bitter gourd （3）Function of McOSC and McP450 genes in bitter gourd
	2021-05
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74554
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	韩雨桐. 苦瓜 McOSC 和 McP450 的功能鉴定及在三萜生物合成中的初步应用, Functional identification of McOSC and McP450 from bitter gourd（Momordica charantia L.）and its preliminary application in triterpenoid biosynthesis[D],2021.

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