基于功能基因组学解析黄曲霉菌降解聚氨酯分子机理的初步研究

	基于功能基因组学解析黄曲霉菌降解聚氨酯分子机理的初步研究; Functional genomics reveals polyurethane biodegradation by Aspergillus flavus
	张世杰
导师	许建初
摘要	Polyurethane biodegradation by fungi is an environmentally friendly end-of-life management for plastic wastes. In this study, a new species of fungus, Amphibambusa hongheensis sp. nov., and Aspergillus flavus G10, a polyurethane-degradable strain of which the polyurethane degradability of Aspergillus flavus has been reported since 2012, have been found at the Honghe Center for Mountain Futures. Firstly, we analyzed and identified the new species A. hongheensis based on the morpho- molecular approach, and the results showed that the new species was visible as black circular or ellipsoid spots on host surface, belonging to the Cainiaceae family. Phylogenetic analysis showed that this new species was most closely related to Amphibambusa bambusicola. Subsequently, we tested the degradation of polyurethane by A. hongheensis and A. flavus G10, two species of fungi from the same area. The results showed that A. hongheensis had no ability to degrade polyurethane, while A. flavus G10 could effectively degrade polyurethane. A. flavus is an aerobic saprophytic fungus, and its secondary metabolite aflatoxin seriously harms the safety of cash crops and the health of human and animals. With the rapid development of omics studies such as genomics and transcriptome, important progress has been made in the study of secondary metabolism of A. flavus by using these omics techniques. However, studies on the molecular mechanism of A. flavus degrading plastics by using these omics techniques are very rare. In order to further study the molecular mechanism of fungal degradation of polyurethane, in this study, the genome and transcriptome sequencing of A. flavus G10 were carried out after the previous experiment on the degradation of polyester polyurethane by A. flavus G10. Firstly, we reported a high-quality genome of A. flavus G10 by Illumina and Single Molecular Real-Time sequencing technology. The genome of this fungus is about 37 Mb in size, 2.0 Mb in N50 scaffold length, 49.9% in GC content and comprises 11,399 predicted coding genes, encoding 1,893 secreted proteins. Moreover, the comparative genomic analysis confirmed that A. flavus G10 and Aspergillus oryzae are closely related species, which was consistent with the results of previous studies. Furthermore, the transcriptional profiling of different groups of this strain showed that the genes for some crucial enzymes related to PU degradation were upregulated, including esterase and so on. This study found a new fungus species A. hongheensis, and test its no degradation of the polyurethane ability. After that, the genomic and transcriptomic analysis of A. flavus G10 provide evidence that A. flavus G10 has the capacity of PU degradation and involve in the potential metabolic pathway of degradation. This work also has important implications for future industry of plastic wastes processing.
	2021-05
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74546
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	张世杰. 基于功能基因组学解析黄曲霉菌降解聚氨酯分子机理的初步研究, Functional genomics reveals polyurethane biodegradation by Aspergillus flavus[D],2021.

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