滇牡丹降血糖活性成分的比较分析和挖掘

	滇牡丹降血糖活性成分的比较分析和挖掘; Antidiabetic Comparison and Active Constituents of Paeonia delavayi
	黄骞
导师	耿长安
摘要	Paeonia suffruticosa and Paeonia delavayi are two famous medicinal plants in Paeoniaceae. Our previous investigation revealed that different parts of P. suffruticosa showed potent antidiabetic effects by inhibiting α-glucosidase, PTP1B, and DPP4, whereas the chemical difference and antidiabetic property of P. delavayi had not yet been studied. P. delavayi is mainly distributed in the northwestern part of Yunnan, southwestern Sichuan, and southeastern Tibet of China. Its roots are always used as the substitute of P. suffruticosa in the production of crude drugs, with antibacterial, anti-inflammatory and anticoagulant effects. This study was designed to clarify the chemical constituents and antidiabetic potency of P. delavayi by LCMS-IT-TOF analysis and enzyme inhibition on α-glucosidase, PTP1B, TCPTP, and DPP4. By interpretation of their UV absorptions and MS/MS fragmentations, and/or comparison with reference samples, a total of 57 constituents including 10 monoterpene glycosides, 15 flavonoids, eight triterpenoids, seven galloyl glucoses, five gallic acids, six N-containing compounds, two acetophenones, and four other types of compounds were identified from the different parts of P. delavayi. PLS-DA analysis showed that monoterpenoids and acetophenone were mainly present in root bark and root core; triterpenoids were mainly distributed in root core; flavonoids and N-containing compounds were mainly distributed in stems and leaves; galloyl glucose and gallic acid derivatives were widely distributed in four parts of P. delavayi. Different parts of P. delavayi (200 μg/mL) showed obvious inhibition on α-glucosidase and PTP1B with the inhibition rates of 81.2%-98.5%, but moderate inhibition on TCPTP and DPP4 (19.5%-34.9%). In particular, root bark, root core and stem showed obvious inhibition against α-glucosidase with IC50 values of 0.12, 0.10 and 0.17 μg/mL, higher than that of leaves (IC50 = 1.5 μg/mL). The stems exhibited the highest activity on PTP1B (IC50 = 97.5 μg/mL), then followed with root bark (IC50 = 107.0 μg/mL), leaves (IC50 = 162.0 μg/mL), and root core (IC50 = 151.0 μg/mL). Five types of characteristic constituents in Paeonia plants were further studied for their inhibition on α-glucosidase, PTP1B, TCPTP and DPP4. At the concentration of 200 μM, monoterpene glycosides and acetophenone were inactive to the four enzymes; gallic acid displayed moderate inhibitory effects on PTP1B and TCPTP, with inhibition rates of 52.1% and 67.1%; flavonoids showed different inhibitory activities on four enzymes: luteolin could inhibit α-glucosidase, PTP1B, and TCPTP with IC50 values of 94.6, 136.3, and 157.3 μM; quercetin inhibited PTP1B, TCPTP, and DPP4 with IC50 values of 195.0, 182.0, and 147.3 μM; apigenin 4'-O-β-D-glucopyranose had an IC50 value of 182.5 μM against DPP4; akebonic acid showed significant inhibition on α-glucosidase and PTP1B, with IC50 values of 73.5 and 57.8 μM. In order to clarify the antidiabetic constituents in P. suffruticosa
	2021-05
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74517
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	黄骞. 滇牡丹降血糖活性成分的比较分析和挖掘, Antidiabetic Comparison and Active Constituents of Paeonia delavayi[D],2021.

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