单羽苏铁的群体遗传学研究

	单羽苏铁的群体遗传学研究; Population genetics of Cycas simplicipinna
	王祎晴
导师	龚洵
摘要	Cycas simplicipinna (T. Smitinand) K. Hill belongs to genus Cycas L. in Cycadaceae, which distributed in China, Thailand, Laos and Vietnam. Previous research on C. simplicipinna only collected molecular materials and specimens, and this is unable to analyse the habitat characteristics, population size and age structure of C. simplicipinna, resulting in a lack of comprehensive and effective protection. In this study, field surveys was conducted on the recorded distribution sites of C. simplicipinna by referring to specimens and literatures in China. A total of 57 507 individuals in 15 populations were investigated, with about 16 719.61 m2 distribution areas. And the age structure of each population was stable and the seedling regeneration was in good condition. Sixteen populations (11 populations in China, one population in Thailand, two populations in Laos, one population in Vietnam) of C. simplicipinna were selected to study genetic diversity, genetic structure, haplotype divergence time and population dynamic history by using four chloroplast sequences (psbB-psbH, trnL-trnF, psbM-trnD, trnS-trnG), three single copy nuclear genes (GTP, PHYP, PPRC) and eight microsatellite markers. Combined field investigations and endangered causes of C. simplicipinna analyses , protection strategies were proposed. The results of population genetics showed that C. simplicipinna had high genetic diversity at species level. Haplotype diversity of chloroplast sequences was higher than nuclear genes (cpDNA: 0.914, GTP: 0.753, PHYP: 0.811, PPRC: 0.792), but nucleotide polymorphism of chloroplast sequences was significantly lower than nuclear genes (cpDNA: 1.25×10-3, GTP: 2.9×10-3, PHYP: 2.71×10-3, PPRC: 1.71×10-3). The genetic diversity of C. simplicipinna was low at population level. There was significant genetic differentiation among populations (Fst: cpDNA, 0.969; GTP, 0.793; PHYP, 0.805; PPRC, 0.765; SSR: 0.267). The results of Mantel Test based on cpDNA, nuclear genes and SSR markers all showed that there was no significant correlation between geographical distance and genetic distance. Genetic structure of C. simplicipinna based on different molecular markers were different. According to clustering results of SSR, 15 C. simplicipinna populations were divided into four clades (Clade I - IV). Clade I include LU, LUA, THE and VIE populations distributed in Vietnam, Thailand and Laos. Clade II contains MM, ML, MY and SY populations distributed in Mengla county, Yunnan Province, China. Clade III contains NZD, NBH, LC, SMG and YZ populations distributed in Xishuangbanna and Puer City, Yunnan Province, China. Clade IV contains BB and DW populations in Lincang city, Yunnan province, China. These suggested that C. simplicipinna contain four evolutionary significant units. Based on cpDNA, 15 populations of C. simplicipinna were divided into two clades, one containing 11 populations from Yunnan and the other containing four populations from foreign countries (Laos, Thaila
	2021-06
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74515
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	王祎晴. 单羽苏铁的群体遗传学研究, Population genetics of Cycas simplicipinna[D],2021.

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