DNA条形码技术在疣柄牛肝菌属真菌分类中的应用

	DNA条形码技术在疣柄牛肝菌属真菌分类中的应用; Application of DNA Barcoding in the Identification of Fungal Species in Leccinum
	孟欣
导师	李艳春
摘要	DNA barcoding technology is to quickly and accurately identify species using one or more standard DNA sequences with enough mutation and easily amplified in organisms. In this study, it applied to the species identification and discovery of the genus Leccinum. Species in Leccinum are ectomychorhizal fungi and economically and ecologically important. Species in Leccinum are diverse in morphology, complicate in structure, and wide in ecological niches. The relationships among species of the genus are very complicated. Currently, there are following scientific problems in this genus: (1) the identification of species in this genus from China need to be verified and reconfirmed, especially those referenced to the names of European or North American species; (2) The species diversity of this genus in China is seriously scanty; and a large number of new species need to be described urgently; (3) the relationships among species within the genus are largely unclear. Accordingly, based on large-scale sampling of representative samples of the genus, this study emploied integrated and inter-disciplinary approaches including morphological-anatomical characterization, molecular phylogenetic analyses of multigene nucleotide sequences, and field ecological observations to study the phylogeny of Leccinum. The goals of this study are to elucidate phylogenetic relationships among species within the genus, to put forward delimitation standards of the species, to document and illustrate the new species in China, and then to evaluate the seven DNA barcode candidates in order to select standard DNA barcode to identify species in this genus. The main results are summarized as follows: 1. Classification and molecular phylogeny of Leccinum In this study, 180 specimens were studied, and seven fragments were selected, namely ribosomal large subunit (nrLSU), internal internal transcribed spacer (ITS) encoding ribosomal RNA, mitochondrial ATP synthase (atp6), translation elongation factor 1-α(tef1-α), gene encoding RNA polymerase II first two large subunits (rpb1, rpb2) and mitochondrial ribosomal small subunit (mtSSU), and a total of 905 related sequences of this genus were obtained. The phylogenetic tree of Leccinum was constructed using the separate and combined analysis of seven gene fragments, and the species diversity of the genus was analyzed by combining morphological studies. Our studies show that: (1) Leccinum formed a monophyletic group with high support, and it can be divided into two major clades, which represented two sections, namely L. sect. Luteoscabra and L. sect. Leccinum. (2) Through this study, the species number of Leccinum in China was increased from 19 to 29. There are about 18 species of L. sect. Leccinum (including 10 known species and 8 new species) and 11 species of L. sect. Luteoscabra (including 6 known species and 5 new species). Greatly enrich the diversity and uniqueness of the genus. (3) There are two species complexes in L. sect. Leccinum,
	2021-05
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74491
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	孟欣. DNA条形码技术在疣柄牛肝菌属真菌分类中的应用, Application of DNA Barcoding in the Identification of Fungal Species in Leccinum[D],2021.

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孟欣-DNA条形码技术在疣柄牛肝菌属真菌（24840KB）	学位论文		限制开放	CC BY-NC-SA	请求全文