KIB OpenIR  > 昆明植物所硕博研究生毕业学位论文
蓖麻化成生和子理究矮化形成的生理和分子机理研究; The physiological and molecular mechanisms of dwarfism formation in castor bean
Thesis Advisor刘爱忠
Abstractn present study, the two castor bean varieties, CBt with tall stem and CBd with dwarf stem were designed as parents. Firstly, we surveyed the plant height difference between parents on the levels of cytology and physiology. Then, we constructed a F2 recombinant population consisted of 330 recombinant individuals using CBt and CBd as parents. Based on F2 population, we analyzed the distribution of plant height for F2 progenies, designed a tall bulk and a dwarf bulk which consisted of 28 extremely tall F2 progenies and extremely dwarf F2 progenies, and performed BSA analysis. Next, the key candidate gene was screened based on the BSA analysis and the RNA-Seq data of xylem (Xy), cambia (Ca) and phloem (Ph) tissues of CBt and CBd. The function of key candidate gene was searched using subcellular localization and yeast heterologous expression system, and the relationship between the key candidate gene and the variations on the levels of histomorphology and physiology, was also exploration. At we known, our study is the only research, which identified the key gene involving castor plant height regulation via to BSA strategy, and further carried out function study to it. It provides novel insights into the molecular mechanisms of dwarfism in castor bean, and contributes solid theoretical basis to implement castor bean dwarfing breeding. The research achievements consisted of three parts. 1. Cytological and physiological factors affecting castor plant height Firstly, we assessed the plant height and traits related to plant height in a macromorphologic level to CBt and CBd, and found plant height (PH), vertical height of the secondary branching (VHSB), height of primary raceme (HPR), number of node on main stem (NN), diameter of main stem (DMS), and average length of internodes on main stem (ALI) in CBt were significant more than CBd excluding number of node on main stem (NN). Then we calculated the correlation coefficient (r) among plant height and other traits based on the phenotypic data of 330 recombinant individuals in F2 population derived from parents. We found ALI and DMS exhibited the two biggest r with plant height (r = 0.8443 and 0.7762). The inspection within Xy, Ca and Ph tissues between CBt and CBd in a level of cytology, showed a more cell number and a bigger cell size within three tissues of CBt, and a thicker cell wall within the Xy of CBt, were the causes for CBt having longer ALI and thicker DMS than CBd. In physiologic level, the more cellulose, hemicellulose and lignin contents within the Xy of CBt were found, it resulted in the cell wall thickness variation within xylem fibers and vessels between CBt and CBd. Plant hormones’ measurement based on the apical buds which is a main tissue of hormone biosythnese, showed the concentration of IAA and GAs (GA1, GA3, GA4, GA12, GA19 and GA44) were inverse to plant height variation between CBt and CBd, and the zeatin was no significant difference between two varieties. Itsuggested the biosy
Document Type学位论文
Recommended Citation
GB/T 7714
王再青. 蓖麻化成生和子理究矮化形成的生理和分子机理研究, The physiological and molecular mechanisms of dwarfism formation in castor bean[D],2021.
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