KIB OpenIR  > 昆明植物所硕博研究生毕业学位论文
乌脚绿竹组培诱导开花与开花调控机制的研究; In vitro flowering induction and flowering regulation mechanism of Bambusa odashimae
Thesis Advisor郭振华
AbstractFlowering is one of the most important and complicated developmental stage in the life of seed plants, which is the basis of their reproduction. However, woody bamboo, as an important forest resource, shows distinctive features in flowering, including a long time at the vegetative growth, unpredictable flowering time and rapid death after flowering. These not only result in great losses for the ecological environment and the bamboo forest industry, but also make it difficult to perform the scientific research on bamboo flowering due to lack of bamboo flowering materials. In the present study, Bambusa odashimae was used to induce flowering in vitro and a system for regrowth after flowering was established, which shortened the vegetative growth time. The samples of B. odashimae at different stages including before flowering and after flowering were sequenced through the full-length transcriptome and RNA-sequencing. Comparing with the flowering of Dendrocalamus latiflorus Munro,the genes related to flowering transition and their regulatory networks were investigated, which elucidated the genetic regulation mechanism of flowering transition in B. odashimae. The present results provide significant information for understanding the unique flowering in bamboo. The main results are as follows: (1)Induction of flowering in vitro of two bamboo In the present study, the induced-flowering in vitro systems were developed for two bamboos (B. odashimae and Dendrocalamus barbatus). They were both cultured on Murashige and Skoog (MS) medium supplemented with cytokinin (CTK). For B. odashimae, axillary buds of single-node stem segments were used as explants and 90% of the buds could flower within five months, then auxin (auxin, AUX) can be used to induce and restore vegetative growth after flowering. The flowers in vitro induced from B. odashimae showed a complete structure but sterile pollens, which failed to produce fertile seeds. The seeds from D. barbatus were used as explants. The clone plants were obtained and then induced to flower. After eight months of induction, limited flower buds were obtained and most of the flowers were abnormal. Therefore, the system of induced-flowering in vitro of B. odashimae was used in the following study. (2)Full-length Transcriptome Analysis Pacbio was first used to sequence B. odashimae at different growth stages and the full-length transcriptomic data were obtained. There were 260,289 full length non-chimeric (FLNC) transcripts and after removal of redundant sequences, a total of 82,458 non-redundant transcripts were produced. Through analyzing the transcripts of non-redundant transcripts, 1,004 alternative splice variants were obtained and 20,001 SSR and 4,713 lncRNA were predicted. According to eight different annotation platforms, a total of 76,792 non-redundant transcripts were annotated, Through analysis of the full-length transcriptomic data, the N50 was 1699 bp and the GC content was 50.46% while through analysis of
Document Type学位论文
Recommended Citation
GB/T 7714
王玉娇. 乌脚绿竹组培诱导开花与开花调控机制的研究, In vitro flowering induction and flowering regulation mechanism of Bambusa odashimae[D],2020.
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