DNA条形码在硬皮马勃属真菌物种鉴别中的应用

	DNA条形码在硬皮马勃属真菌物种鉴别中的应用; The application of DNA barcoding technology in species identification of Scleroderma
	周吕蓉
导师	葛再伟
摘要	Species of Scleroderma form ectomycorrhizal associations with various plants, playing an important role in ecosystem. Many of these Scleroderma species also have medicinal functions such as hemostasis, detumescence, bacteriostasis, insecticidal, antiviral and anti-inflammatory. While most species within Scleroderma are not edible, Scleroderma yunnannse, a peculiar species described from Yunnan, has been considered to be delicious, thus has been widely collected and sold in the local market. Thus far, except S. yunnannse, Scleroderma specimens collected from China are ascribed to 12 species originally described from other parts of the world. Whether these species distributed in China is still lack of molecular evidence. Besides, identification of Scleroderma species is largely dependent on observations of their microscopic characters such as basidiospores, making it rather difficult for researchers other than mycologist. In recent years, the development of fungal DNA barcoding has provided a useful method for the identification of fungi, and the internal transcribed spacer (ITS) has been recommended as a formal barcode for fungi. However, how well ITS performs as a barcode in Scleroderma remains untested. Searching for suitable DNA barcodes in Scleroderma could provide a basis for rapid species identification to facilitate the researchers without professional taxonomic knowledge on Scleroderma. In this study, using samples of Scleroderma collected in recent years, 7 candidate DNA regions (ITS, nrLSU, tef1-α, atp6, rpb1, rpb2, mcm7) were evaluated to investigate how well they discriminate the species of Scleroderma as potential DNA barcodes. (1) Based on the sequences generated, the 127collections have been identified as 13 phylogenetic species. Among these, 5 are putative new species that need further morphological study to determine whether they are indeed new taxa, and detailed descriptions of three novel taxa are provided; 4 species (Scleroderma leave, S. aurantium, S. suthepense and S. xanthochoum) are new records for China; and 4 species (S. areolatum, S. bovista, S. sinamaliense and S. yunnanense) are previously known species recorded in China. Among the 26 collections of S. yunnanense, 23 of these were collected from Yunnan Province (the only distribution area previously thought), and the other 3 collections were from Anhui Province (1 collection) and Guangdong province (2 collections) respectively, indicating a much wider distribution area than that previously thought. (2) According to the rate of PCR and sequencing successful rate, universality of primers, the fragment length, variation of sequences, reliability of multiple sequence alignment, DNA barcoding gap and species resolution ability, ITS performed the best based on sequences data of the 11 species represented by 69 collections thus can be the primary fungal barcode marker for Scleroderma. While nrLSU, tef1-α and atp6 have intraspecific variation and interspecific variation partia
	2020-05
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74227
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	周吕蓉. DNA条形码在硬皮马勃属真菌物种鉴别中的应用, The application of DNA barcoding technology in species identification of Scleroderma[D],2020.

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