类皱叶香茶菜的化学成分及其生物活性研究

	类皱叶香茶菜的化学成分及其生物活性研究; Studies on the Chemical Constituents and Bioactivity of Isodon rugosiformis
	张勤钰
导师	孙汉董
摘要	Isodon rugosiformis Hand.-Mazz. Hara is distributed throughout the northwest of the Yunnan Province and the west of the Sichuan Province, People’s Republic of China, belonging to the genus Isodon of the family Labiatae. The species is obviously different from other ones of the same genus: unusual plant morphology with some red leaves. So far, there has been no research reports about the chemical constituents of this species, except the chemical study by our group, which mainly indicated the main constituents to be ent-kauranoids and abietanoids, including three unprecedented skeletons. In addition, the bioactivity screening showed that the novel abietane, rugosiformin D, partially inhibited rabbit platelet aggregation induced by arachidonic acid. Therefore, this kind of plant is worth thorough research. In order to search for structurally novel and bioactive diterpenoids, further study was undertaken on I. rugosiformis. Chemical investigations of this species resulted in the isolation and structure determination of 51 compounds (of which 17 new ones): including 46 diterpenoids (of which 16 were new); 3 triterpenoids; 1 new sesquiterpenoid; 1 lignan. Among them, the diverse 46 diterpenoids included seven types: (i) 6,7:8,15-diseco-6,8-cyclo-ent-kauranoids (isorugosiformins A–F); (ii) 7,20-epoxy-ent-kauranoids (isorugosiformins G–I); (iii) 6,7-seco-ent-kauranoids (isorugosiformins J–K); (iv) 7,20:19,20-diepoxy-ent-kaurane (isorugosiformin L); (v) abietane (isorugosiformin M); (vi) ent-abietane (isorugosiformin N); (vii) ent-kaurane dimers (isorugosiformins O–P). The absolute configurations of the five new compounds were determined by the single-crystal X-ray diffraction. Some of diterpenoids were screened for cytotoxic activity against the HL-60, SMMC-7721, A-549, MCF-7, and SW-480 human tumor cell lines, and evaluated for anti-inflammatory activities against LPS-induced nitric oxide (NO) production in RAW 264.7 macrophages, and screened for immunosuppressive activity. Through the secondary metabolites’ investigations of this species, not only enriched the diterpene structure type of the genus Isodon, at the
	2020-05
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74217
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	张勤钰. 类皱叶香茶菜的化学成分及其生物活性研究, Studies on the Chemical Constituents and Bioactivity of Isodon rugosiformis[D],2020.

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