Knowledge Management System of Kunming Institute of Botany,CAS
菟丝子介导寄主间氮磷系统性信号的研究; Parasite dodder enables transfer of systemic nitrogen and phosphate signals between host plants | |
张井雄 | |
导师 | 吴建强 |
摘要 | Parasitism is an important ecological phenomenon. About 1% (4000-5000 species) angiosperm plants are parasitic. Dodders (Cuscuta spp., Convolvulaceae) are parasitic plants with worldwide distribution. Dodders can simultaneously parasitize two or more adjacent hosts, forming dodder-connected plant clusters. Previous studies demonstrated that dodder could transfer herbivory-induced systemic signals between different hosts. Nitrogen (N) and phosphorus (Pi) deficiency are common challenges to plants. To date, it is yet unclear whether dodder transfers N or Pi-systemic signals between hosts grown in N or Pi-heterogeneous soil. In this thesis, transcriptome and methylome analyses were carried out to investigate whether dodder (Cuscuta campestris) transfers N-systemic signals between N-replete and N-depleted cucumber (Cucumis sativus) hosts. It was found that N-systemic signals from the N-deficient cucumber plants were rapidly translocated through dodder to the N-replete cucumber plants. Unexpectedly, certain systemic signals were also transferred from the N-replete to N-depleted cucumber hosts. We demonstrated that these systemic signals can regulate large transcriptome and DNA methylome changes in the recipient hosts. Importantly, N stress also induced many long-distance mobile mRNA transfers between dodder and hosts, and the bilateral N-systemic signaling between N-replete and N-depleted hosts had a strong impact on the inter-plant mobile mRNAs. Our 15N labeling experiment indicated that under N-heterogeneous conditions, N-systemic signals from the N-deficient cucumber hosts did not change the N uptake activity of the N-replete cucumber hosts; however, in plant clusters comprising dodder-connected cucumber and soybean (Glycine max) plants, if the soybean plants are N-starved, cucumber plants exhibit increased N uptake activity. Thus, dodder facilitates plant-plant communications under N-stress conditions by enabling extensive bilateral N-systemic signaling between different hosts. In this thesis, we also show that dodder can also mediate bidirectional phosphate systemic signals between hosts. Transcriptome profiling experiments indicated that dodder was able to transfer certain systemic signals bidirectionally between the Pi-depleted and Pi-replete hosts. Transcriptome changes were detected from 6 h to 48 h suggesting that the Pi systemic signals were produced and transmitted by dodder at 6 h. In dodder, 60-379 DEGs were detected. Thus, dodder seems to be more sensitive to hosts’ Pi status than N. Similar to the N system, more mobile mRNAs from hosts to dodder than dodder to hosts were observed. In cucumber, the expression level of mobile mRNAs were higher than the non-mobile mRNAs. GO analysis on the mobile mRNAs specifically transferred from a host under Pi stress condition shown that many N and Pi metabolism terms are enriched, indicating that N and Pi may share certain signaling components. Furthermore, chlorophyll and protein contents of hosts |
2020-11 | |
文献类型 | 学位论文 |
条目标识符 | http://ir.kib.ac.cn/handle/151853/74215 |
专题 | 昆明植物所硕博研究生毕业学位论文 |
推荐引用方式 GB/T 7714 | 张井雄. 菟丝子介导寄主间氮磷系统性信号的研究, Parasite dodder enables transfer of systemic nitrogen and phosphate signals between host plants[D],2020. |
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