KIB OpenIR  > 昆明植物所硕博研究生毕业学位论文
青藏高原传统栽培作物芜菁抗冻的分子机制; Antifreeze molecular mechanism of traditional cultivated turnip (Brassica rapa var. rapa) in the Tibetan Plateau
Thesis Advisor杨永平
AbstractIn the agricultural area of the Qinghai-Tibet Plateau, there is a remarkable diurnal temperature variation during plant growing season, which is not only one of the limiting factors affecting crop yield, but also one of the driving forces for crops to adapt to regional climate. In order to better understand how crops response to and adapt to low temperature in the Tibetan Plateau, there is a regional need to screen resistant genetic resources and develop stress resistant crop varieties. Turnip (Brassica rapa var. rapa) is a traditional tap-root crop cultivated by Tibetans in the Tibetan Plateau and Yi people in southwest China and used as food, fodder and medicine. According to the results of turnip common garden, a cold-resistant landrace, KTRG-B49, from Tibetan Autonomous Region, was identified and used as materials to reveal the turnip anti-freezing mechanism in the Tibetan Plateau. Based on the transcriptome analysis, we further studied the effect of CBL-CIPK family on the response to low temperature conditions and screened the transcription factors regulated by CBL-CIPK to explore the anti-freeze molecular mechanism of turnip. The key findings are as follows: (1) Transcriptome analysis of Tibetan turnip KTRG-B49 under freezing treatments The transcriptome analysis of Tibetan turnip KTRG-B49 under freezing treatment suggested that differentially expressed genes (DEGs) encoded by calcium sensors protein family, and the protein kinase family were significantly expressed, including calmodulin (CaMs, 2 DEGs), CaM-like proteins (CMLs, 6 DEGs), calcineurin B-like proteins (CBLs, 5 DEGs), calcium dependent protein kinase (CDPKs, 7 DEGs), MAP kinase kinase kinase (MAPKKKs, 7 DEGs), CBL interacting serine/threonine protein kinases (CIPKs, 14 DEGs) and mitogen activated protein kinases (MPKs, 2 DEGs)). The results indicated that the calcium signal pathway played a role in the anti-freeze process of Tibetan turnip. (2) Genome-wide identification and functional analysis of the calcineurin B-like protein and calcineurin B-like protein-interacting protein kinase gene families in turnip The calcineurin B-like protein (CBL)-CBL-interacting protein kinase (CIPK) complex were used as a primary component in calcium sensors that perceives various stress signals. In the present study, 19 and 51 members of the BrrCBL and BrrCIPK genes, respectively, were first identified in turnip and phylogenetically grouped into three and two distinct clusters, respectively. The expansion of these two gene families was mainly attributable to segmental duplication. Moreover, the differences in expression patterns in quantitative real-time PCR in different tissues, different developmental stages and different treatment conditions, as well as interaction profiles in the yeast two-hybrid assay, suggested the signal systems composed of different CBLs and CIPKs had different response modes to different adversity during long-term evolution in turnip, and these differences revealed
Document Type学位论文
Recommended Citation
GB/T 7714
尹欣. 青藏高原传统栽培作物芜菁抗冻的分子机制, Antifreeze molecular mechanism of traditional cultivated turnip (Brassica rapa var. rapa) in the Tibetan Plateau[D],2020.
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