JA浸种增强植物抗虫性及MeJA对马铃薯生长与产量的影响

	JA浸种增强植物抗虫性及MeJA对马铃薯生长与产量的影响; Enhanced Herbivore Resistance by Jasmonic Acid Seed Priming in Arabidopsis and Effects of Foliar Sprayed Methyl Jasmonate on Potato Minituber Growth and Yield
	杨天宇
导师	王蕾
摘要	Treating seeds with jasmonic acid (JA) improves herbivore resistance of plants, however, the molecular mechanisms underling this process are poorly understood. In this study, we found that the Arabidopsis seedlings grown from seeds soaked in 3 mmol/L JA solution exhibited enhanced resistance against the generalist herbivore, Spodoptera litura: larvae grown on these plants gained 14.5% more weight compared with those grown on the untreated plants. In response to mechanical wounding, plants grown from JA-treated seeds accumulated higher contents of jasmonoyl-isoleucine (JA-Ile), which is a bioactive jasmonate, and higher levels of glucosinolates, which are anti-herbivore secondary metabolite. RNA-seq data showed that JA seed treatment enhanced the transcription of many JA pathway genes, and weakly down-regulated genes function in DNA methylation, histone modification, and chromatin modification. These results imply that JA seed treatment enhances plant resistance against herbivory by changing epigenetic modifications of certain JA pathway genes. In order to explore the effect of exogenous application of JA on potato yield and growth, Yunshu 505 was used as the experimental material to determine the JA contents in the four early stages of tuber development. Then the methyl jasmonate (MeJA) foliar spraying, which was gradually transform to JA, was used to study the effect of MeJA on potato growth and tuber yield. The results of liquid chromatography-mass spectrometry (LC-MS) showed the accumulation of JA increased during the process of potato tuber enlargement. During the production of minitubers, 100 μmol/L MeJA was sprayed on the leaves at different frequencies. There were four spray frequency groups, called the control group (CK); 2 times /day (T1); 1 time/day (T2); 1 time/2 days (T3). Then we measured and counted the changes of plant traits, tuber traits and yield. The results of the field experiment showed that, compared with CK, the stem thickness of T2 treatment increased by 36.1%, and the chlorophyll content of T1 group decreased by 20.1%. In addition, there were no significant differences between the groups about these plant traits like the plant's leaf color, stem color, flower color, plant type and other growth traits and tuber size regularity, potato shape, skin color, flesh color, potato skin type, sprout eye depth, cracking rate, hollow rate and other tuber characteristics. However, the survival rates of plants in the T1, T2, T3, and CK groups were 45.6%, 100%, 100%, and 87.3%, respectively; the equivalent yields of the T1, T2, and T3 groups increased by -15.6%, 8.8%, and 12.1%, respectively, compared with the CK group. Comprehensive analysis showed that the accumulation level of JA in potatoes increased during the early stage of tuber formation. Foliar spraying of 100 μmol/L MeJA at a frequency of 1 time/2 days increased the yield of potato minitubers without affecting growth.
	2020-08
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74207
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	杨天宇. JA浸种增强植物抗虫性及MeJA对马铃薯生长与产量的影响, Enhanced Herbivore Resistance by Jasmonic Acid Seed Priming in Arabidopsis and Effects of Foliar Sprayed Methyl Jasmonate on Potato Minituber Growth and Yield[D],2020.

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