茵陈蒿的化学成分及其代谢研究

	茵陈蒿的化学成分及其代谢研究; Chemical constituents of Artemisia capillaris and the metabolism study
	肖瑶
导师	李飞
摘要	Cholestatic liver injury is various reseaons-induced bile acid excretion disorder and bile acids retention. The toxic bile acid sataied in liver caused liver injury, and this liver injury may further progresse to hepatic fibrosis and liver cirrhosis. Hyperlipidemia is a disease with an abnormally high level of lipids in the blood, result from lipid metabolism disorder, and it may trigger serious diseases, such as atherosclerosis, coronary heart disease, stroke and other cardiovascular and cerebrovascular diseases, which represent a substantial global threat to human health. However, currently the effective drugs for the treatment of these two diseases are relatively limited or have strong side-effects. Regarding to the importance of natural products in drug development, it is meaningful to discover new hepatoprotective and hypolipidemic compounds from natural sources. Artemisia capillaris is one of the two major plant sources of traditional Chinese medicine Yinchen, belonging to the genus Artemisia in the Compositae family. Yinchen, a traditional Chinese medicine using to treat liver diseases, is the treatment of choice for jaundice. Pharmacological studies have shown that some extracts and chemical components of Artemisia capillaris have broad pharmacological activities, like hepatoprotective effect, choleretic effect, as well as anti-inflammatory, anti-tumor, anti-oxidation, pathogenic microorganisms, hypolipidemic pharmacological activity. In this work, the EtOAc extract of A. capillaris has been isolated using several chromatographic separation methods (silica gel CC, sephedex LH-20, MCI, semi-preparative HPLC), yielding 29 compounds, including five coumarins, five phenylpropionic acids, eight phenolic acids, two lignans, two terpenes, two alkaloids, two steroids, one flavonoid, one enyne and two aliphatic compounds, of which 14 compounds were isolated and identified from A. capillaris for the first time. The hepatoprotective effect of compounds on the hepatocellular injury induced by deoxycholic acid (DCA), a toxic bile acid, was evaluated on mice primary hepatocytes. Nine compounds, scopoletin, 7-methoxycourmain, ethyl caffeoate, dendroarboreol B, vanillin, 4-(1-methoxylethyl) phenol, hydroquinone, 2-methoxy-5-(α-hydroxyethyl) phenol, ethyl 3,4-dihydroxybenzoate were found to restore DCA-reduced hepatocyte vitality decrease. The hypolipidemic activity of some compounds was preliminarily evaluated on sodium oleate-induced HepG2 lipid accumulation model and 3T3-L1 preadipocytes. It was found that ethyl caffeate and caffeic acid could inhibit sodium oleate-induced HepG2 cell lipid accumulation at a concentration of 50 μM, the inhibition rates were 22.3±4.47% and 15.8±7.40%, respectively, and the inhibition rate of the positive drug fenofibrate was 21.4±5.78%. At a concentration of 50 μM, ethyl caffeate and caffeic acid inhibited the differentiation and lipid accumulation of 3T3-L1 preadipocytes by 18.4±4.30% and 11.4±0.61%, respectively. Coum
	2020-08
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74196
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	肖瑶. 茵陈蒿的化学成分及其代谢研究, Chemical constituents of Artemisia capillaris and the metabolism study[D],2020.

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