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| 月季连续开花调控候选基因WD40-60功能探究; Identification and characterization of WD40-60, a WD40 family transcription factor, in regulation of continuous flowering trait of roses | |
| 孙弋博 | |
| Thesis Advisor | 胡金勇 |
| Abstract | Roses (Rosa sp., Rosaceae) are pivotal horticultural plants that featuring key morphological traits. Rosa chinensis 'Old Blush' (OB) is one of the most important parents for rose domestication due to its CF behavior. Another founder genotype, R. wichuriana 'Basye's Thornless', flowers only once a year (OF). Recently, the available of genome sequences for a doubled-haploid OB and a diploid BT offers an important opportunity to dissect genetically the molecular basis underlying continuous flowering trait. With comparative genomics and molecular genetic approaches, in this thesis, we identified the molecular genetic bases responsible for CF behavior and examined the potential roles of the WD40-60, a member of WD40 family of transcriptional regulator, in regulation of this trait. The main findings are: 1. Rose WD40-60 gene is a candidate underlying continuous flowering trait. we identified two close quantitative trait loci (QTL) strongly linked with CF trait. Within these QTL, one WD40-60 gene, which encodes an important transcription factor of circadian clock regulation in Arabidopsis thaliana, is selected for further molecular investigation. 2. Enormous sequence variation presents in WD40-60s between OB and BT. At the protein level, overexpression of both RcWD40-60 and RwWD40-60 in Col-0 leads to a significant later flowering, while the overexpression of both proteins in Arabidopsis double mutant, complements the flowering to wild type levels. However, under short-day conditions, overexpression of the two genes can significantly but differentially regulate the expression of the clock genes AtPRR7, AtPRR9 and AtCCA1. 3. Expression variation between RcWD40-60 and RwWD40-60 associates with flowering behavior of OB and BT. The promoter sequences -2 Kb upstream of start codon may act as activation region for both genes, while the -5 ~ -2 Kb region mainly serves as repressive function. Furthermore, the -5 ~ -2 Kb regions contains cis-regulatory-elements specific for expression in phloem and stomata cells. These sequence differences may contribute to the flowering behavior variation between the two rose genotypes. 4. WD40 family diversifies significantly in roses. We identify 187 WD40 genes and RcWD40-60 has three homologs. we find a duplication of COP1-like genes in roses. The two COP1-likes diverge in both their protein sequences and their protein interaction partners (i.e., HY5 and SPA4). In summary, we identify the QTL and propose WD40-60 as one of the key candidates regulating CF trait. Though further functional tests are in progresses, our data in this thesis provide for the first time a candidate regulator involved in responses to circadian clock variation. Our findings are important not only for the understanding of molecular mechansisms for regulation of continuous flowering trait itself but also for the tracing of the origin and domestication history of China roses. |
| 2020-11 | |
| Document Type | 学位论文 |
| Identifier | http://ir.kib.ac.cn/handle/151853/74179 |
| Collection | 昆明植物所硕博研究生毕业学位论文 |
| Recommended Citation GB/T 7714 | 孙弋博. 月季连续开花调控候选基因WD40-60功能探究, Identification and characterization of WD40-60, a WD40 family transcription factor, in regulation of continuous flowering trait of roses[D],2020. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 孙弋博-孙弋博毕业论文2020.12.2（6926KB） | 学位论文 | 限制开放 | CC BY-NC-SA | Application Full Text | ||
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