热激蛋白HSP101的新功能探索

	热激蛋白HSP101的新功能探索; Exploration on novel functions of HEAT SHOCK PROTEIN HSP101
	秦凤
导师	李唯奇
摘要	Heat shock proteins (HSPs) are a class of proteins that are used to deal with environmental stress such as high temperature. According to the molecular weight, HSPs can be divided into Hsp100, Hsp90, HSP70, HSP60 and small heat shock protein (sHSP) subfamilies. HSP101, a member of Hsp100 / ClpB subfamily, is a star molecular chaperone for plants to response to heat stress. When plants are under heat stress, HSP101 can help to depolymerize protein aggregates and refold proteins, thus enhancing the thermotolerance of plants. Although the biochemical and physiological functions of HSP101 on thermotolerance have been fully studied, there still remain many scientific problems about HSP101 to be further explored. For example, whether HSP101 has more biological functions under nonstress conditions and how to perform them. In this paper, the model plant Arabidopsis thaliana was used to mainly study the regulation of HSP101 on flowering time and seed longevity under nonstress conditions, by physiology, molecular biology, genetics, immunohistochemistry and other methods. The main conclusions are as follows: Firstly, HSP101 positively regulates flowering in Arabidopsis under nonstress conditions. HSP101 did not affect the morphological structure of Arabidopsis， but influenced the flowering time. Compared with the Col-0 wild type, the flowering time of HSP101 transgenic line (35S::HSP101-GFP) was earlier, and that of functional deficient mutant (hot1-1, hot1-3) was delayed. And the flowering time of complementation lines (hot1-1 / 35S:: 35S::HSP101-GFP, hot1-3 / 35S::HSP101-GFP) was earlier than that of the mutant lines. Among the 20 key genes of known flowering regulating pathways, expression of 8 genes showed significant differences among different HSP101 genotypes, involving various flowering regulating pathways. Among them, FLC and SVP were dramatically up-regulated in the HSP101 mutants. The effect of HSP101 on transcriptional regulation of multiple genes was integrated by the downstream floral integrators (FT, SOC1, LFY, AP1) of the flowering regulating network, thus affecting flowering time. Physiological experiments showed that HSP101 mutants flowered later in both long and short days, and responded normally to vernalization, GA, temperature and sucrose treatments. The results indicated that HSP101 promoted flowering mainly through the autonomous pathway. The promotion effect of HSP101 on flowering depended on FLC, the mutants of FLC and SVP could repress the late flowering phenotype of HSP101 mutants. HSP101 was widely distributed in the cell membrane, cytoplasm and nucleus, and was more concentrated in the nucleus under nonstress condition. In conclusion, HSP101 promotes flowering in Arabidopsis mainly through the autonomous pathway, relying on FLC. Secondly, HSP101 positively regulates seed storage longevity in Arabidopsis. Compared with the seeds of Col-0 wild type, that of HSP101 functional deletion mutant lines (hot1-1, hot1-3) were more sensit
	2020-08
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74167
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	秦凤. 热激蛋白HSP101的新功能探索, Exploration on novel functions of HEAT SHOCK PROTEIN HSP101[D],2020.

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