牡丹的化学与生物活性比较和降血糖活性成分发掘

	牡丹的化学与生物活性比较和降血糖活性成分发掘; Chemical and Biological Comparison of Paeonia suffruticosa and its Antidiabetic Constituents
	潘秧
导师	耿长安
摘要	Paeonia suffruticosa Andr. (Mudan) is a famous medicinal and ornamental plant widely cultivated in China. In the traditional Chinese medicine (TCM) system, only the root bark (Moutan Cortex) is used in the production of crude drugs. The present study aimed to systematically compare the chemical profiles and biological activities of different parts of P. suffruticosa (root bark, root core, old stems, young stems, and leaves) by LCMS-IT-TOF analysis and bioassay in vitro. A total of 55 constituents involving 15 monoterpene glycosides, 13 galloyl glucose, seven gallic acid derivatives, four acetophenones, six flavonoids, and ten other compounds were characterized according to their UV absorptions, MS/MS fragmentation, and comparing with reference compounds. The MS fragmentation rues of monoterpene glycosides were discussed. Quantitative analysis demonstrated gallic acid mainly in leaves (5439.1 ± 90.2 μg/g), paeoniflorin mainly in root bark (16378.9 ± 195.4 μg/g), root core (17130.2 ± 125.9 μg/g), and leaves (16682.9 ± 181.2 μg/g). Benzoylpaeoniflorin and paeonol mainly in root bark (2360.1 ± 41.4, 10142.3 ± 252.5 μg/g) and root core (2249.5 ± 21.7, 10441.6 ± 363.9 μg/g) were the main constituents in P. suffruticosa. The chemical constituents of root bark and root core were similar and obviously different from the stems and leaves. In this paper, the pharmacological activities of different parts of P. suffruticosa were compared by determining their agonistic effects on four kinds of neurotransmitter receptors (MT1, MT2, 5-HT1A and 5-HT2C) and inhibitory effects on three enzymes (α-glucosidase, PTP1B and DPP IV). The old and young stems showed high active on four neurotransmitter receptors, and especially on MT2 receptor with agonistic rates of 128.7% and 140.6%; the root bark and root core showed moderate activity on MT1, MT2 and 5-HT1A, but inactive to 5-HT2C receptor; the leaves only exhibited activity on 5-HT1A receptor. All the five parts showed obvious inhibition on α-glucosidase and PTP1B (>90%, 400 μg/mL), but were inactive to DPP IV. The extraction rate of root bark was 35.4% ± 2.18, which was two times higher than that of root core (18.4% ± 0.81), supporting root bark as a traditional medicine. In this study, the leaves of P. suffruticosa were revealed with antidiabetic potency in vitro. In order to clarify the active constituents in leaves, various chromatographic column chromatography methods were applied to obtain 24 compounds, involving 13 triterpenes (1-12), two monoterpene glycosides (13, 14), and ten other compounds (15-24). Two new noroleanane-type triterpenoids were characterized as paeonenoides I-J (1-2), according to their HRESIMS, 1D and 2D NMR, UV, IR spectra. Compounds 3, 5, 6, 9 and 10 showed PTP1B inhibitory effects with the inhibition rates of 77.30%, 51.56%, 74.45%, 89.67% and 94.28% at 400 μM. The review section summarized the research progress of Paeoniaceae plants in the past ten years, mainly the chemical constituent
	2020-05
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74166
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	潘秧. 牡丹的化学与生物活性比较和降血糖活性成分发掘, Chemical and Biological Comparison of Paeonia suffruticosa and its Antidiabetic Constituents[D],2020.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
潘秧-定稿-Pan-28f1e77db-（9367KB）	学位论文		限制开放	CC BY-NC-SA	请求全文