菟丝子与寄主间蛋白质大规模转运研究

	菟丝子与寄主间蛋白质大规模转运研究; A study on the large-scale protein transfer between parasitic plant Cuscuta and its host plants
	刘念
导师	吴建强
摘要	About 1% of angiosperms (ca. 4500 species) are parasitic plants, which have unique physiology, ecology, and evolutionary histories. Parasitic plants evolved to have a special organ, the haustorium, through which parasitic plants fuse with the host vasculature systems to obtain water and nutrients, which support the growth of parasitic plants. Dodders belong to the genus Cuscuta (Convolvulaceae) and there are about 200 dodder species. Their roots and leaves are highly vestigial and have become invisible. Dodders are totally dependent on hosts to survive. Dodder use haustoria to invade into host plants’ stem and form vascular connections with them. Therefore, macromolecular proteins are likely to move long-distance between dodder and host plants through these vascular connections, and some transferred signaling proteins may affect the growth and physiology of dodder and host plants. Using dodder and its hosts (such as Arabidopsis, soybean, tobacco, cucumber and wild tomato) as the experimental systems, and by means of several techniques such as label-free peptide quantitation proteome analyses, bioinformatics, molecular biology and biochemistry, we intended to explore the protein transfer between dodder and host plants and the functions of inter-plant protein transfer. The main findings are listed as the following: 1. Large amount of proteins are translocated between dodder and its hosts We collected the stems, leaves, and seeds of dodder and host plants for label-free peptide quantitation proteome analyses. It was found that hundreds to more than one thousand proteins were translocated between the stems of dodder and those of Arabidopsis or soybean. Large numbers of these inter-plant mobile proteins were also found in the seeds of dodder and soybean plants. Moreover, when dodder bridge connected Arabidopsis and soybean plants, the exchange of hundreds of proteins between these two hosts occurred. The quantities of transferred protein were estimated to represent 2.23% to 16.68% of the proteomes of these plants. Some transcription factors and resistant-related proteins such as R proteins and P450s were found to be translocated. 2. Some transferred proteins may have biological functions in recipient plants According to the proteomic analyses, at least 12% of mobile proteins retained more than 50% of their abundance in their native plants, after they travelled to the foreign plants. Thus, these proteins may perform their biological roles in recipient plants. Six reporter proteins eGFP-GUS, GUS, LUC, AOS, PAT, and EPSPS were found to be translocated from the host Arabidopsis or soybean plants to dodder, and eGFP-GUS and AOS were found to be translocated between Arabidopsis and soybean through dodder connections. These reporter proteins retained their biological functions after long-distance transport. Therefore, inter-plant exchange of proteins may have physiological and ecological significance. 3. Transferred proteins are mostly likely not translated
	2020-06
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74156
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	刘念. 菟丝子与寄主间蛋白质大规模转运研究, A study on the large-scale protein transfer between parasitic plant Cuscuta and its host plants[D],2020.

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